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|a Nano-Bio Probe Designand Its Application for Biochemical Analysis; Preface; Contents; 1 Introduction; 2 Nanomaterial-Enhanced Fluorescence Polarization and Its Application; 2.1…Principles of Fluorescence Polarization; 2.2…Sensitive Detection of Hg2+ by Fluorescence Polarization Enhanced by Gold Nanoparticle; 2.3…DNAzyme-Self-Assembled Gold Nanoparticles for Determination of Cu2+ and Pb2+ Using Fluorescence Polarization Assay; 2.4…Peptide-Functionalized Spherical Polyelectrolyte Nanobrushes for Real-Time Sensing of Protease Activity; References
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|a 3 Versatile Graphene-Based Nano-Bio Probe Design and Its Application3.1…Introduction; 3.2…Graphene-DNA Molecular Beacon Design and its Application; 3.3…Graphene-Protein Nanoprobe Design and its Application; 3.4…Other Graphene-Based Nanoprobes and Their Applications; References; 4 Metal Nanoparticles-Based Colorimetric Probe Design and Its Application; 4.1…Introduction; 4.2…Sensitive and Rapid Colorimetric Detection of Biological Thiols Using Nucleotide-Stabilized Silver Nanoparticles; 4.3…Colorimetric Chiral Recognition of Enantiomers Using Nucleotide-Capped Silver Nanoparticles
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|a 4.4…Colorimetric Assay for Phthalates Using UTP-Modified Gold Nanoparticles Cross-Linked by Copper (II)4.5…Multiplexed Analysis of Ag+ and Hg2+ Using Oligonucleotide-Metal Nanoparticle Conjugates; References; 5 Metal-Nanoclusters-Based Luminescent Probe Design and Its Application; 5.1…Introduction; 5.2…Metal-Nanoclusters-Based Luminescent Probes for Nucleic Acid Detection; 5.3…Metal-Nanoclusters-Based Luminescent Probes for Metal Ions Detection; 5.4…Metal-Nanoclusters-Based Luminescent Probes for Cellular Labeling or Imaging; References;
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|a In this volume, Prof. Ye and his coworkers propose and review the concept of nano-bio probe design for biochemical analysis on the basis of their recent published works. A unique biochemical analysis technology based on fluorescence polarization enhanced by nanoparticles is described here with successful applications in environmental monitoring, rapid and sensitive sensing protease activity and high-throughput screening of inhibitors. Furthermore, they introduce a versatile molecular beacon (MB)-like probe for the multiplex sensing of targets such as sequence-specific DNA, protein, metal ions and small molecule compounds based on the self-assembled biomolecule-graphene conjugates. Besides, some colorimetric and luminescence probes utilizing metal nanoparticles for biochemical applications are also presented, such as chiral enantiomer discrimination and separation, environmental monitoring, clinic diagnosis and etc.
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