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|a Molecular Farming in Plants: Recent Advances and Future Prospects
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|a Molecular Farming in Plants:Recent Advances and Future Prospects; Preface; Contents; Editors; Contributors; Chapter 1: Molecular Farming in Plants: An Overview; 1.1 Introduction; 1.2 Overview of Plant-Based Expression Systems; 1.2.1 Transgenic Plants; 1.2.2 Chloroplast Transformed Plants; 1.2.3 Transient Expression; 1.2.4 Plant Cell Suspension Cultures; 1.3 Approaches for Increasing Heterologous Protein Accumulation in Plants; 1.3.1 Boosting Transcription; 1.3.2 Boosting Translation; 1.3.3 Subcellular Targeting; 1.3.4 Fusion Protein-Based Approaches
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|a 2.4 Treatment of Type 1 Diabetes with Transgenic Plants Expressing Diabetes-Associated Autoantigens2.4.1 Induction of Oral Tolerance by Mucosal Administration of Transgenic Plants Expressing GAD; 2.4.2 Induction of Oral Tolerance by Mucosal Administration of Transgenic Plants Expressing Insulin; 2.5 Treatment of Arthritis with Transgenic Plants Expressing Arthritis-Associated Autoantigens; 2.5.1 Induction of Oral Tolerance by Mucosal Administration of Transgenic Plants Expressing Type II-Collagen Peptides
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|a Chapter 3: Molecular Farming Using Bioreactor-Based Plant Cell Suspension Cultures for Recombinant Protein Production3.1 Introduction; 3.2 Recombinant Protein Production Using Plant Cell Suspension Cultures; 3.2.1 Features of Plant Cell Suspension Cultures for Recombinant Protein Production; 3.2.2 Method to Establish Transgenic Plant Cell Suspension Cultures; 3.2.3 Example of Transgenic Nicotiana benthamiana Cell Suspension Cultures; 3.2.3.1 Plant Cell Transformation and Media; 3.2.3.2 Transgenic Plant Cell Suspension Cultures and Media
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|a 1.4 Technologies and Strategies for Recovery of Recombinant Proteins from Plants1.4.1 Affinity Fusion-Based Protein Purification; 1.4.2 Non-affinity-Based Protein Purification; 1.4.3 Soybean Agglutinin Fusion as a Novel System for High-Level Expression and Purification of Plant-Made Proteins; 1.5 Conclusions; References; Chapter 2: Induction of Oral Tolerance to Treat Autoimmune and Allergic Diseases by Using Transgenic Plants; 2.1 Introduction; 2.2 Oral Tolerance and Its Therapeutic Potential in Autoimmunity and Allergy; 2.3 Transgenic Plants as a Novel Strategy for Oral Tolerance Induction
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|a 2.5.2 Induction of Oral Tolerance by Mucosal Administration of Transgenic Plants Expressing Mycobacterial Hsp652.6 Induction of Oral Tolerance to Treat Allergic Diseases with Transgenic Plants Expressing Allergens; 2.6.1 Induction of Oral Tolerance by Mucosal Administration of Transgenic Plants Expressing a Full-Length Antigen; 2.6.2 Induction of Oral Tolerance by Mucosal Administration of Transgenic Rice Expressing Allergen-Specific T Cell Epitopes; 2.7 Treatment of Inflammatory Bowel Disease by Oral Administration of Transgenic Plants Expressing Interleukin-10; 2.8 Conclusions; References
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|a Molecular farming in plants is a relatively young subject of sciences. As plants can offer an inexpensive and convenient platform for the large-scale production of recombinant proteins with various functions, the driven force from the giant market for recombinant protein pharmaceuticals and industrial enzymes makes this subject grow and advance very quickly. To summarize recent advances, current challenges and future directions in molecular farming, international authorities were invited to write this book for researchers, teachers and students who are interested in this subject. This book, wi
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|a Molecular farming in plants is a relatively young subject of sciences. As plants can offer an inexpensive and convenient platform for the large-scale production of recombinant proteins with various functions, the driven force from the giant market for recombinant protein pharmaceuticals and industrial enzymes makes this subject grow and advance very quickly. To summarize recent advances, current challenges and future directions in molecular farming, international authorities were invited to write this book for researchers, teachers and students who are interested in this subject. This book, wi
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