Details der Publikation - Super-resolved linear fluorescence localization microscopy using photostable fluorophores

Super-resolved linear fluorescence localization microscopy using photostable fluorophores : a virtual microscopy study / Udo Birk, Aleksander Szczurek, Christoph Cremer

Current approaches to overcome the conventional limit of the resolution potential of light microscopy (of about 200 nm for visible light), often suffer from non-linear effects, which render the quantification of the image intensities in the reconstructions difficult, and also affect the quantification of the biological structure under investigation. As an attempt to face these difficulties, we discuss a particular method of localization microscopy which is based on photostable fluorescent dyes. The proposed method can potentially be implemented as a fast alternative for quantitative localization microscopy, circumventing the need for the acquisition of thousands of image frames and complex, highly dye-specific imaging buffers. Although the need for calibration remains in order to extract quantitative data (such as the number of emitters), multispectral approaches are largely facilitated due to the much less stringent requirements on imaging buffers. Furthermore, multispectral acquisitions can be readily obtained using commercial instrumentation such as e.g. the conventional confocal laser scanning microscope..

Medienart:	E-Artikel

Erscheinungsjahr:	21 July 2017 2017
Erschienen:	21 July 2017

Enthalten in:	Zur Gesamtaufnahme - volume:404
Enthalten in:	Optics communications - 404(2017), Seite 42-50

Sprache:	Englisch

Beteiligte Personen:	Birk, Udo J., 1971- [VerfasserIn] Szczurek, Aleksander, 1989- [VerfasserIn] Cremer, Christoph, 1944- [VerfasserIn]

Links:	Volltext Volltext

Themen:	Linear fluorescence microscopy Photostable dyes SMLM Super-resolution fluorescence microscopy

Anmerkungen:	Available online 21 July 2017 Gesehen am 14.08.2018

Umfang:	9

doi:	10.1016/j.optcom.2017.06.078

funding:
Förderinstitution / Projekttitel:

PPN (Katalog-ID):	1580018246

Internformat


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Super-resolved linear fluorescence localization microscopy using photostable fluorophores : a virtual microscopy study / Udo Birk, Aleksander Szczurek, Christoph Cremer

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