In vivo confocal microscopy of inflammatory cells in the corneal subbasal nerve plexus in patients with different subtypes of anterior uveitis / Andreea S. Postole, Alexandra B. Knoll, Gerd U. Auffarth, Friederike Mackensen

PURPOSE: Previously we could show increased numbers and densities of dendritic-like cells (DLCs) in the subbasal nerve plexus of the central cornea in patients with herpetic anterior uveitis (HAU). Now we aimed to explore these and other inflammatory cells seen in this layer in different subtypes of anterior uveitis using in vivo confocal microscopy. METHODS: Consecutive eyes of patients with different types of anterior uveitis, HAU, Fuchs' uveitis syndrome (FUS), juvenile idiopathic arthritis (JIA) and human leucocyte antigen (HLA)-B27-related anterior uveitis were examined in vivo with the combination of Heidelberg Retina Tomograph II/III and Rostock Cornea Module. The contralateral eye was used as control. Inflammatory cells were defined on the basis of their morphology: type 1 (DLCs) and type 2 (cell bodies lacking dendrites). Frequencies were evaluated statistically in each group. RESULTS: The difference between means of type 1 cells density of affected eyes in all four groups was significant (one-way analysis of variance (ANOVA) p=0.039). The difference between means of type 1 cell densities of affected eyes in patients with HAU (96.8±44.2 cells/mm(2), n=10) and that of patients with FUS (46.4±38.7 cells/mm(2), n=17) was significant (Tukey's post hoc p=0.025), whereas the difference between patients with HAU and JIA (53.3±34.5 cells/mm(2), n=7) and patients with HAU and HLA-B27 (63.1±59.2 cells/mm(2), n=10) was not significant (Tukey's post hoc p=0.181 and 0.300). In contrast, the following means resulted from the evaluation of type 2 cells: the difference between means of affected eyes in all four groups was not significant (one-way ANOVA p=0.185). Density means difference of patients with HAU (44.9±22.6 cells/mm(2), n=5) and that of FUS (20.0±11.0 cells/mm(2), n=2) and that of patients with JIA (56.0±18.3 cells/mm(2), n=2) and that of HLA-B27 (36.1±24.1 cells/mm(2), n=5) was not significant (Tukey's post hoc p=0.302, 0.877 and 0.739). The contralateral eye of all patient groups showed also an inflammatory cell infiltrate of lesser extent. CONCLUSIONS: The high density and morphology of DLCs in the central cornea of patients with HAU assessed by confocal microscopy supports the clinical diagnosis of HAU especially when compared with patients with FUS but not when compared with patients with JIA or HLA-B27. CLINICAL RELEVANCE: This study suggests that the non-invasive confocal microscopy of the cornea is capable of supporting a clinical diagnosis in patients with uveitis..

Medienart:

E-Artikel

Erscheinungsjahr:

28 January 2016

2016

Erschienen:

28 January 2016

Enthalten in:

Zur Gesamtaufnahme - volume:100

Enthalten in:

British journal of ophthalmology - 100(2016), 11, Seite 1551-1556

Sprache:

Englisch

Beteiligte Personen:

Postole, Andreea-Silvia, 1988- [VerfasserIn]
Knoll, Alexandra, 1985- [VerfasserIn]
Auffarth, Gerd U. [VerfasserIn]
Mackensen, Friederike, 1974-2016 [VerfasserIn]

Links:

Volltext [kostenfrei]

Themen:

Cell Count
Cornea
Female
HLA-B27 Antigen
Humans
Imaging
Infection
Inflammation
Male
Microscopy, Confocal
Middle Aged
Nerve Fibers
Nerve Net
Ophthalmic Nerve
ROC Curve
Uveitis, Anterior

Anmerkungen:

Gesehen am 20.11.2017

Umfang:

6

doi:

10.1136/bjophthalmol-2015-307429

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

156550402X

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