Practical methods for biocatalysis and biotransformations 2

Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 2 is a "how-to" guide that focuses on the practical applications of enzymes and strains of microorganisms that are readily obtained or derived from culture collections. The sources of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. This second volume - which can be used on its own or in combination with the first volume - concentrates on new applications and new enzyme families reported since the first volume. Contents include: introduction to recent developments and future needs in biocatalysts and synthetic biology in industry reductive amination enoate reductases for reduction of electron deficient alkenes industrial carbonyl reduction regio- and stereo- selective hydroxylation oxidation of alcohols selective oxidation industrial hydrolases and related enzymes transferases for alkylation, glycosylation and phosphorylation C-C bond formation and decarboxylation halogenation/dehalogenation/heteroatom oxidation tandem and sequential multi-enzymatic syntheses Practical Methods for Biocatalysis and Biotransformations 2 is an essential collection of biocatalytic methods for chemical synthesis which will find a place on the bookshelves of synthetic organic chemists, pharmaceutical chemists, and process R&D chemists in industry and academia..

PRACTICAL METHODS FOR BIOCATALYSIS AND BIOTRANSFORMATIONS 2 -- Contents -- List of Contributors -- Abbreviations -- 1 Biocatalysis in the Fine Chemical and Pharmaceutical Industries -- 1.1 Introduction -- 1.2 Biotrans Outsourcing - AstraZeneca -- 1.3 Biotrans Trends - Lonza -- 1.4 Biocatalysis in the Pharma Environment -- 1.5 Industrial Use of Hydrolases -- 1.6 Industrial Biooxidation and Reduction -- 1.7 Industrial Application of Transaminases - Cambrex -- 1.8 Biocatalyst Discovery and Improvement -- 1.9 From Pathway Engineering to Synthetic Biology -- 1.10 Prioritization of Future Biocatalysis and Synthetic Biology Needs -- 1.11 Concluding Remarks -- Acknowledgements -- References -- 2 Reductive Amination -- 2.1 ω-Transaminases - Useful Biocatalysts for Chiral Amine Synthesis -- 2.2 Preparative Scale Production of a Bulky-Bulky Chiral Amine Using an Engineered Transaminase -- 2.3 Synthesis of Optically Pure Amines Employing ω-Transaminases -- 2.4 A Fast, Sensitive Assay and Scale-Up of ω-Transaminase Catalysed Reactions -- 2.5 Asymmetric Synthesis of L-3-Hydroxyadamantylglycine Using Branched Chain Aminotransferase -- 2.6 Asymmetric Reduction of Aryl Imines Using Candida parapsilosis ATCC 7330 -- 3 Enoate Reductases for Reduction of Electron Deficient Alkenes -- 3.1 Asymmetric Bioreduction of Activated Alkenes Using Ene-Reductases from the Old Yellow Enzyme Family -- 3.2 Efficient Baker's Yeast Mediated Reduction with Substrate Feeding Product Removal (SFPR) Technology: Synthesis of (S)-2-Alkoxy-3-Aryl-1-Propanols -- 3.3 Asymmetric Reduction of (4S)-(+)-Carvone Catalyzed by Enoate Reductases (ERs) Expressed by Non-Conventional Yeast (NCY) Whole Cells -- 3.4 Preparation of Enantiomerically Pure Citronellal Enantiomers Using Alkene Reductases..

Medienart:	E-Book

Erscheinungsjahr:	2012
Erschienen:	Chichester, UK: J. Wiley ; 2012

Reproduktion:	Online-Ausg. ; LaVergne,Tenn. ; Hampshire ; Myilibrary ; Online-Ressource ; MyiLibrary
Weitere Ausgaben:	Druckausg.: Practical methods for biocatalysis and biotransformations ; 2
Enthalten in:	Zur Gesamtaufnahme - 2

Sprache:	Englisch

Links:	Volltext [lizenzpflichtig] Cover

ISBN:	978-1-119-99139-7

Themen:	Biocatalysis Biotransformation Biotransformation (Metabolism) Electronic books Enzymes Enzymes ; Biotechnology Organic compounds Organic compounds ; Synthesis

Anmerkungen:	Includes bibliographical references and index. - Description based on print version record

Umfang:	330 S. ; Ill., graph. Darst

Förderinstitution / Projekttitel:

PPN (Katalog-ID):	1453117342