Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction
Abstract Background The emergence of SARS-CoV-2 variants is a current public health concern possibly impacting COVID-19 disease diagnosis, transmission patterns and vaccine effectiveness.Objectives To describe the SARS-CoV-2 lineages circulating early pandemic among samples with S gene dropout and characterize a novel mutation in receptor-binding domain (RBD) of viral spike protein.Study design Adults and children older than 2 months with signs and symptoms of COVID-19 were prospectively enrolled from May to October 2020 in Porto Alegre, Brazil. All participants performed RT-PCR assays for diagnosing SARS-CoV-2, samples with S gene dropout and Ct < 30 (cycle threshold) were submitted to whole genome sequencing (WGS), and homology modeling and physicochemical properties analysis were performed.Results 484/1,557 participants tested positive for SARS-CoV-2. The S gene dropout was detected in 7.4% (36/484) as early as May, and a peak was observed in early August. WGS was performed in 8 samples. The B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic. The RBD novel mutation (Y380Q) was found in one sample occurring simultaneously with C379W and V395A, and the B.1.91 lineage in the spike protein.Conclusion Mutations in the SARS-CoV-2 spike region were detected early in the COVID-19 pandemic in Southern Brazil, regarding the B.1.1.28, B.1.91 and B.1.1.33 lineages identified. The novel mutation (Y380Q) with C379W, modifies important RBD properties, which may interfere with the binding of neutralizing antibodies (CR3022, EY6A, H014, S304).Highlights <jats:list list-type="bullet">Characterization of novel mutation (Y380Q) in RBD of SARS-CoV-2 spike proteinThe Y380Q and C379W modify important properties in the SARS-CoV-2 RBD regionThe RBD mutations may interfere with the binding of neutralizing antibodiesThe B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic.
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 25. Mai Zur Gesamtaufnahme - year:2022 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Sauthier Sartor, Ivaine Tais [VerfasserIn] |
|---|
| Links: |
|---|
| doi: |
10.1101/2021.09.10.21262695 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI032579306 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI032579306 | ||
| 003 | DE-627 | ||
| 005 | 20230429092944.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 210915s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2021.09.10.21262695 |2 doi | |
| 035 | |a (DE-627)XBI032579306 | ||
| 035 | |a (biorXiv)10.1101/2021.09.10.21262695 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | |a 570 |q DE-84 | |
| 100 | 1 | |a Sauthier Sartor, Ivaine Tais |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Abstract Background The emergence of SARS-CoV-2 variants is a current public health concern possibly impacting COVID-19 disease diagnosis, transmission patterns and vaccine effectiveness.Objectives To describe the SARS-CoV-2 lineages circulating early pandemic among samples with S gene dropout and characterize a novel mutation in receptor-binding domain (RBD) of viral spike protein.Study design Adults and children older than 2 months with signs and symptoms of COVID-19 were prospectively enrolled from May to October 2020 in Porto Alegre, Brazil. All participants performed RT-PCR assays for diagnosing SARS-CoV-2, samples with S gene dropout and Ct < 30 (cycle threshold) were submitted to whole genome sequencing (WGS), and homology modeling and physicochemical properties analysis were performed.Results 484/1,557 participants tested positive for SARS-CoV-2. The S gene dropout was detected in 7.4% (36/484) as early as May, and a peak was observed in early August. WGS was performed in 8 samples. The B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic. The RBD novel mutation (Y380Q) was found in one sample occurring simultaneously with C379W and V395A, and the B.1.91 lineage in the spike protein.Conclusion Mutations in the SARS-CoV-2 spike region were detected early in the COVID-19 pandemic in Southern Brazil, regarding the B.1.1.28, B.1.91 and B.1.1.33 lineages identified. The novel mutation (Y380Q) with C379W, modifies important RBD properties, which may interfere with the binding of neutralizing antibodies (CR3022, EY6A, H014, S304).Highlights <jats:list list-type="bullet">Characterization of novel mutation (Y380Q) in RBD of SARS-CoV-2 spike proteinThe Y380Q and C379W modify important properties in the SARS-CoV-2 RBD regionThe RBD mutations may interfere with the binding of neutralizing antibodiesThe B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic | ||
| 700 | 1 | |a Varela, Fernanda Hammes |e verfasserin |4 aut | |
| 700 | 1 | |a Meireles, Mariana Rost |e verfasserin |4 aut | |
| 700 | 1 | |a Kern, Luciane Beatriz |e verfasserin |4 aut | |
| 700 | 1 | |a Azevedo, Thaís Raupp |e verfasserin |4 aut | |
| 700 | 1 | |a Tumioto Giannini, Gabriela Luchiari |e verfasserin |4 aut | |
| 700 | 1 | |a da Silva, Mariana Soares |e verfasserin |4 aut | |
| 700 | 1 | |a Demoliner, Meriane |e verfasserin |4 aut | |
| 700 | 1 | |a Gularte, Juliana Schons |e verfasserin |4 aut | |
| 700 | 1 | |a de Almeida, Paula Rodrigues |e verfasserin |4 aut | |
| 700 | 1 | |a Fleck, Juliane Deise |e verfasserin |4 aut | |
| 700 | 1 | |a Zavaglia, Gabriela Oliveira |e verfasserin |4 aut | |
| 700 | 1 | |a Fernandes, Ingrid Rodrigues |e verfasserin |4 aut | |
| 700 | 1 | |a de David, Caroline Nespolo |e verfasserin |4 aut | |
| 700 | 1 | |a Santos, Amanda Paz |e verfasserin |4 aut | |
| 700 | 1 | |a de Almeida, Walquiria Aparecida Ferreira |e verfasserin |4 aut | |
| 700 | 1 | |a Gomes Porto, Victor Bertollo |e verfasserin |4 aut | |
| 700 | 1 | |a Scotta, Marcelo Comerlato |e verfasserin |4 aut | |
| 700 | 1 | |a Vieira, Gustavo Fioravanti |e verfasserin |4 aut | |
| 700 | 1 | |a Spilki, Fernando R. |e verfasserin |4 aut | |
| 700 | 1 | |a Stein, Renato T. |e verfasserin |4 aut | |
| 700 | 1 | |a Polese-Bonatto, Márcia |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2022) vom: 25. Mai |
| 773 | 1 | 8 | |g year:2022 |g day:25 |g month:05 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.diagmicrobio.2022.115636 |z lizenzpflichtig |3 Volltext |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2021.09.10.21262695 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |j 2022 |b 25 |c 05 | ||
| 953 | |2 045F |a 570 | ||