Sperm membrane proteins DCST1 and DCST2 are required for the sperm-egg fusion process in mice and fish
Abstract The process of sperm-egg fusion is critical for successful fertilization, yet the underlying mechanisms that regulate these steps have remained unclear in vertebrates. Here, we show that both mouse and zebrafish DCST1 and DCST2 are necessary in sperm to fertilize the egg, similar to their orthologs SPE-42 and SPE-49 inC. elegansand Sneaky inD. melanogaster. MouseDcst1andDcst2single knockout (KO) spermatozoa are able to undergo the acrosome reaction and show normal relocalization of IZUMO1, an essential factor for sperm-egg fusion, to the equatorial segment. While both single KO spermatozoa can bind to the oolemma, they rarely fuse with oocytes, resulting in male sterility. Similar to mice, zebrafishdcst1KO males are subfertile anddcst2anddcst1/2double KO males are sterile. Zebrafishdcst1/2KO spermatozoa are motile and can approach the egg, but rarely bind to the oolemma. These data demonstrate that DCST1/2 are essential for male fertility in two vertebrate species, highlighting their crucial role as conserved factors in fertilization..
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Preprint| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
bioRxiv.org - (2023) vom: 02. Nov. Zur Gesamtaufnahme - year:2023 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Noda, Taichi [VerfasserIn] |
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| doi: |
10.1101/2021.04.18.440256 |
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| PPN (Katalog-ID): |
XBI020376219 |
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| 245 | 1 | 0 | |a Sperm membrane proteins DCST1 and DCST2 are required for the sperm-egg fusion process in mice and fish |
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| 520 | |a Abstract The process of sperm-egg fusion is critical for successful fertilization, yet the underlying mechanisms that regulate these steps have remained unclear in vertebrates. Here, we show that both mouse and zebrafish DCST1 and DCST2 are necessary in sperm to fertilize the egg, similar to their orthologs SPE-42 and SPE-49 inC. elegansand Sneaky inD. melanogaster. MouseDcst1andDcst2single knockout (KO) spermatozoa are able to undergo the acrosome reaction and show normal relocalization of IZUMO1, an essential factor for sperm-egg fusion, to the equatorial segment. While both single KO spermatozoa can bind to the oolemma, they rarely fuse with oocytes, resulting in male sterility. Similar to mice, zebrafishdcst1KO males are subfertile anddcst2anddcst1/2double KO males are sterile. Zebrafishdcst1/2KO spermatozoa are motile and can approach the egg, but rarely bind to the oolemma. These data demonstrate that DCST1/2 are essential for male fertility in two vertebrate species, highlighting their crucial role as conserved factors in fertilization. | ||
| 650 | 4 | |a Biology |7 (dpeaa)DE-84 | |
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| 700 | 1 | |a Blaha, Andreas |0 (orcid)0000-0002-0995-4228 |4 aut | |
| 700 | 1 | |a Fujihara, Yoshitaka |0 (orcid)0000-0001-8332-3507 |4 aut | |
| 700 | 1 | |a Gert, Krista R. |0 (orcid)0000-0002-5673-5335 |4 aut | |
| 700 | 1 | |a Emori, Chihiro |0 (orcid)0000-0001-8040-2517 |4 aut | |
| 700 | 1 | |a Deneke, Victoria E. |0 (orcid)0000-0002-4404-8706 |4 aut | |
| 700 | 1 | |a Oura, Seiya |0 (orcid)0000-0002-7606-732X |4 aut | |
| 700 | 1 | |a Berent, Sara |0 (orcid)0000-0003-2554-6248 |4 aut | |
| 700 | 1 | |a Kodani, Mayo |4 aut | |
| 700 | 1 | |a Panser, Karin |4 aut | |
| 700 | 1 | |a Cabrera-Quio, Luis Enrique |4 aut | |
| 700 | 1 | |a Pauli, Andrea |0 (orcid)0000-0001-9646-2303 |4 aut | |
| 700 | 1 | |a Ikawa, Masahito |0 (orcid)0000-0001-9859-6217 |4 aut | |
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