SARS-CoV-2 Can Infect Human Embryos
Abstract The spread of SARS-CoV-2 has led to a devastating pandemic, with infections resulting in a range of symptoms collectively known as COVID-19. The full repertoire of human tissues and organs susceptible to infection is an area of active investigation, and some studies have implicated the reproductive system. The effects of COVID-19 on human reproduction remain poorly understood, and particularly the impact on early embryogenesis and establishment of a pregnancy are not known. In this work, we explore the susceptibility of early human embryos to SARS-CoV-2 infection. We note that ACE2 and TMPRSS2, two canonical cell entry factors for SARS-CoV-2, are co-expressed in cells of the trophectoderm in blastocyst-stage preimplantation embryos. Using fluorescent reporter virions pseudotyped with Spike (S) glycoprotein from SARS-CoV-2, we observe robust infection of trophectoderm cells, and this permissiveness could be attenuated with blocking antibodies targeting S or ACE2. When exposing human blastocysts to the live, fully infectious SARS-CoV-2, we detected cases of infection that compromised embryo health. Therefore, we identify a new human target tissue for SARS-CoV-2 with potential medical implications for reproductive health during the COVID-19 pandemic and its aftermath..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 21. März Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Montano, Mauricio [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2021.01.21.427501 |
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| PPN (Katalog-ID): |
XBI019783132 |
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| 520 | |a Abstract The spread of SARS-CoV-2 has led to a devastating pandemic, with infections resulting in a range of symptoms collectively known as COVID-19. The full repertoire of human tissues and organs susceptible to infection is an area of active investigation, and some studies have implicated the reproductive system. The effects of COVID-19 on human reproduction remain poorly understood, and particularly the impact on early embryogenesis and establishment of a pregnancy are not known. In this work, we explore the susceptibility of early human embryos to SARS-CoV-2 infection. We note that ACE2 and TMPRSS2, two canonical cell entry factors for SARS-CoV-2, are co-expressed in cells of the trophectoderm in blastocyst-stage preimplantation embryos. Using fluorescent reporter virions pseudotyped with Spike (S) glycoprotein from SARS-CoV-2, we observe robust infection of trophectoderm cells, and this permissiveness could be attenuated with blocking antibodies targeting S or ACE2. When exposing human blastocysts to the live, fully infectious SARS-CoV-2, we detected cases of infection that compromised embryo health. Therefore, we identify a new human target tissue for SARS-CoV-2 with potential medical implications for reproductive health during the COVID-19 pandemic and its aftermath. | ||
| 700 | 1 | |a Victor, Andrea R. |e verfasserin |4 aut | |
| 700 | 1 | |a Griffin, Darren K. |e verfasserin |4 aut | |
| 700 | 1 | |a Duong, Tommy |e verfasserin |4 aut | |
| 700 | 1 | |a Bolduc, Nathalie |e verfasserin |4 aut | |
| 700 | 1 | |a Farmer, Andrew |e verfasserin |4 aut | |
| 700 | 1 | |a Garg, Vidur |e verfasserin |4 aut | |
| 700 | 1 | |a Hadjantonakis, Anna-Katerina |e verfasserin |4 aut | |
| 700 | 1 | |a Coates, Alison |e verfasserin |4 aut | |
| 700 | 1 | |a Barnes, Frank L. |e verfasserin |4 aut | |
| 700 | 1 | |a Zouves, Christo G. |e verfasserin |4 aut | |
| 700 | 1 | |a Greene, Warner C. |e verfasserin |4 aut | |
| 700 | 1 | |a Viotti, Manuel |e verfasserin |4 aut | |
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