Breadth and function of antibody response to acute SARS-CoV-2 infection in humans

Abstract Serological and plasmablast responses and plasmablast-derived IgG monoclonal antibodies (MAbs) have been analysed in three COVID-19 patients with different clinical severities. Potent humoral responses were detected within 3 weeks of onset of illness in all patients and the serological titre was elicited soon after or concomitantly with peripheral plasmablast response. An average of 13.7% and 13.0% of plasmablast-derived MAbs were reactive with virus spike glycoprotein or nucleocapsid, respectively. A subset of anti-spike (10 of 32) and over half of anti-nucleocapsid (19 of 35) antibodies cross-reacted with other betacoronaviruses tested and harboured extensive somatic mutations, indicative of an expansion of memory B cells upon SARS-CoV-2 infection. Fourteen of 32 anti-spike MAbs, including five anti-RBD, three anti-non-RBD S1 and six anti-S2, neutralised wild-type SARS-CoV-2 in independent assays. Anti-RBD MAbs were further grouped into four cross-inhibiting clusters, of which six antibodies from three separate clusters blocked the binding of RBD to ACE2 and five were neutralising. All ACE2-blocking anti-RBD antibodies were isolated from two patients with prolonged fever, which is compatible with substantial ACE2-blocking response in their sera. At last, the identification of non-competing pairs of neutralising antibodies would offer potential templates for the development of prophylactic and therapeutic agents against SARS-CoV-2..

Medienart:

Preprint

Erscheinungsjahr:

2020

Erschienen:

2020

Enthalten in:

bioRxiv.org - (2020) vom: 21. Okt. Zur Gesamtaufnahme - year:2020

Sprache:

Englisch

Beteiligte Personen:

Huang, Kuan-Ying A. [VerfasserIn]
Tan, Tiong Kit [VerfasserIn]
Chen, Ting-Hua [VerfasserIn]
Huang, Chung-Guei [VerfasserIn]
Harvey, Ruth [VerfasserIn]
Hussain, Saira [VerfasserIn]
Chen, Cheng-Pin [VerfasserIn]
Harding, Adam [VerfasserIn]
Gilbert-Jaramillo, Javier [VerfasserIn]
Liu, Xu [VerfasserIn]
Knight, Michael [VerfasserIn]
Schimanski, Lisa [VerfasserIn]
Shih, Shin-Ru [VerfasserIn]
Lin, Yi-Chun [VerfasserIn]
Cheng, Chien-Yu [VerfasserIn]
Cheng, Shu-Hsing [VerfasserIn]
Huang, Yhu-Chering [VerfasserIn]
Lin, Tzou-Yien [VerfasserIn]
Jan, Jia-Tsrong [VerfasserIn]
Ma, Che [VerfasserIn]
James, William [VerfasserIn]
Daniels, Rodney S. [VerfasserIn]
McCauley, John W. [VerfasserIn]
Rijal, Pramila [VerfasserIn]
Townsend, Alain R. [VerfasserIn]

Links:

Volltext [kostenfrei]

doi:

10.1101/2020.08.28.267526

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

XBI018640338