SCV-2000bp: a primer panel for SARS-CoV-2 full-genome sequencing
ABSTRACT Here we provide technical data for amplifying the complete genome of SARS-CoV-2 from clinical samples using only seventeen pairs of primers. We demonstrate that the СV2000bp primer panel successfully produces genomes when used with the residual total RNA extracts from positive clinical samples following diagnostic RT-PCRs (with Ct in the range from 13 to 20). The library preparation method reported here includes genome amplification of ~1750-2000 bp fragments followed by ultrasonic fragmentation combined with the introduction of Illumina compatible adapters. Using the SCV2000bp panel, 25 complete SARS-CoV-2 virus genome sequences were sequenced from clinical samples of COVID-19 patients from Moscow obtained in late March - early April..
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Preprint| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
bioRxiv.org - (2020) vom: 07. Aug. Zur Gesamtaufnahme - year:2020 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Speranskaya, AS [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2020.08.04.234880 |
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| funding: |
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| PPN (Katalog-ID): |
XBI018503969 |
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| 245 | 1 | 0 | |a SCV-2000bp: a primer panel for SARS-CoV-2 full-genome sequencing |
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| 520 | |a ABSTRACT Here we provide technical data for amplifying the complete genome of SARS-CoV-2 from clinical samples using only seventeen pairs of primers. We demonstrate that the СV2000bp primer panel successfully produces genomes when used with the residual total RNA extracts from positive clinical samples following diagnostic RT-PCRs (with Ct in the range from 13 to 20). The library preparation method reported here includes genome amplification of ~1750-2000 bp fragments followed by ultrasonic fragmentation combined with the introduction of Illumina compatible adapters. Using the SCV2000bp panel, 25 complete SARS-CoV-2 virus genome sequences were sequenced from clinical samples of COVID-19 patients from Moscow obtained in late March - early April. | ||
| 700 | 1 | |a Kaptelova, VV |e verfasserin |4 aut | |
| 700 | 1 | |a Valdokhina, AV |e verfasserin |4 aut | |
| 700 | 1 | |a Bulanenko, VP |e verfasserin |4 aut | |
| 700 | 1 | |a Samoilov, AE |e verfasserin |4 aut | |
| 700 | 1 | |a Korneenko, EV |e verfasserin |4 aut | |
| 700 | 1 | |a Shipulina, OY |e verfasserin |4 aut | |
| 700 | 1 | |a Akimkin, VG |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2020) vom: 07. Aug. |
| 773 | 1 | 8 | |g year:2020 |g day:07 |g month:08 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2020.08.04.234880 |z kostenfrei |3 Volltext |
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