Population Bottlenecks and Intra-host Evolution during Human-to-Human Transmission of SARS-CoV-2
Abstract The emergence of the novel human coronavirus, SARS-CoV-2, causes a global COVID-19 (coronavirus disease 2019) pandemic. Here, we have characterized and compared viral populations of SARS-CoV-2 among COVID-19 patients within and across households. Our work showed an active viral replication activity in the human respiratory tract and the co-existence of genetically distinct viruses within the same host. The inter-host comparison among viral populations further revealed a narrow transmission bottleneck between patients from the same households, suggesting a dominated role of stochastic dynamics in both inter-host and intra-host evolutions.Author summary In this study, we compared SARS-CoV-2 populations of 13 Chinese COVID-19 patients. Those viral populations contained a considerable proportion of viral sub-genomic messenger RNAs (sgmRNA), reflecting an active viral replication activity in the respiratory tract tissues. The comparison of 66 identified intra-host variants further showed a low viral genetic distance between intra-household patients and a narrow transmission bottleneck size. Despite the co-existence of genetically distinct viruses within the same host, most intra-host minor variants were not shared between transmission pairs, suggesting a dominated role of stochastic dynamics in both inter-host and intra-host evolutions. Furthermore, the narrow bottleneck and active viral activity in the respiratory tract show that the passage of a small number of virions can cause infection. Our data have therefore delivered a key genomic resource for the SARS-CoV-2 transmission research and enhanced our understanding of the evolutionary dynamics of SARS-CoV-2..
Medienart: |
Preprint |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
bioRxiv.org - (2021) vom: 15. Dez. Zur Gesamtaufnahme - year:2021 |
---|
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Wang, Daxi [VerfasserIn] |
---|
Links: |
---|
doi: |
10.1101/2020.06.26.173203 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
XBI018233910 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | XBI018233910 | ||
003 | DE-627 | ||
005 | 20230429093918.0 | ||
007 | cr uuu---uuuuu | ||
008 | 200629s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1101/2020.06.26.173203 |2 doi | |
035 | |a (DE-627)XBI018233910 | ||
035 | |a (biorXiv)10.1101/2020.06.26.173203 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | |a 570 |q DE-84 | |
100 | 1 | |a Wang, Daxi |e verfasserin |4 aut | |
245 | 1 | 0 | |a Population Bottlenecks and Intra-host Evolution during Human-to-Human Transmission of SARS-CoV-2 |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a Abstract The emergence of the novel human coronavirus, SARS-CoV-2, causes a global COVID-19 (coronavirus disease 2019) pandemic. Here, we have characterized and compared viral populations of SARS-CoV-2 among COVID-19 patients within and across households. Our work showed an active viral replication activity in the human respiratory tract and the co-existence of genetically distinct viruses within the same host. The inter-host comparison among viral populations further revealed a narrow transmission bottleneck between patients from the same households, suggesting a dominated role of stochastic dynamics in both inter-host and intra-host evolutions.Author summary In this study, we compared SARS-CoV-2 populations of 13 Chinese COVID-19 patients. Those viral populations contained a considerable proportion of viral sub-genomic messenger RNAs (sgmRNA), reflecting an active viral replication activity in the respiratory tract tissues. The comparison of 66 identified intra-host variants further showed a low viral genetic distance between intra-household patients and a narrow transmission bottleneck size. Despite the co-existence of genetically distinct viruses within the same host, most intra-host minor variants were not shared between transmission pairs, suggesting a dominated role of stochastic dynamics in both inter-host and intra-host evolutions. Furthermore, the narrow bottleneck and active viral activity in the respiratory tract show that the passage of a small number of virions can cause infection. Our data have therefore delivered a key genomic resource for the SARS-CoV-2 transmission research and enhanced our understanding of the evolutionary dynamics of SARS-CoV-2. | ||
700 | 1 | |a Wang, Yanqun |e verfasserin |4 aut | |
700 | 1 | |a Sun, Wanying |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Lu |e verfasserin |4 aut | |
700 | 1 | |a Ji, Jingkai |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Zhaoyong |e verfasserin |4 aut | |
700 | 1 | |a Cheng, Xinyi |e verfasserin |4 aut | |
700 | 1 | |a Li, Yimin |e verfasserin |4 aut | |
700 | 1 | |a Xiao, Fei |e verfasserin |4 aut | |
700 | 1 | |a Zhu, Airu |e verfasserin |4 aut | |
700 | 1 | |a Zhong, Bei |e verfasserin |4 aut | |
700 | 1 | |a Ruan, Shicong |e verfasserin |4 aut | |
700 | 1 | |a Li, Jiandong |e verfasserin |4 aut | |
700 | 1 | |a Ren, Peidi |e verfasserin |4 aut | |
700 | 1 | |a Ou, Zhihua |e verfasserin |4 aut | |
700 | 1 | |a Xiao, Minfeng |e verfasserin |4 aut | |
700 | 1 | |a Li, Min |e verfasserin |4 aut | |
700 | 1 | |a Deng, Ziqing |e verfasserin |4 aut | |
700 | 1 | |a Zhong, Huanzi |e verfasserin |4 aut | |
700 | 1 | |a Li, Fuqiang |e verfasserin |4 aut | |
700 | 1 | |a Wang, Wen-jing |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Yongwei |e verfasserin |4 aut | |
700 | 1 | |a Chen, Weijun |e verfasserin |4 aut | |
700 | 1 | |a Zhu, Shida |e verfasserin |4 aut | |
700 | 1 | |a Xu, Xun |e verfasserin |4 aut | |
700 | 1 | |a Jin, Xin |e verfasserin |4 aut | |
700 | 1 | |a Zhao, Jingxian |e verfasserin |4 aut | |
700 | 1 | |a Zhong, Nanshan |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Wenwei |e verfasserin |4 aut | |
700 | 1 | |a Zhao, Jincun |e verfasserin |4 aut | |
700 | 1 | |a Li, Junhua |e verfasserin |4 aut | |
700 | 1 | |a Xu, Yonghao |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2021) vom: 15. Dez. |
773 | 1 | 8 | |g year:2021 |g day:15 |g month:12 |
856 | 4 | 0 | |u https://doi.org/10.3389/fmed.2021.585358 |z lizenzpflichtig |3 Volltext |
856 | 4 | 0 | |u http://dx.doi.org/10.1101/2020.06.26.173203 |z kostenfrei |3 Volltext |
912 | |a GBV_XBI | ||
912 | |a SSG-OLC-PHA | ||
951 | |a AR | ||
952 | |j 2021 |b 15 |c 12 | ||
953 | |2 045F |a 570 |