Single-cell screening of SARS-CoV-2 target cells in pets, livestock, poultry and wildlife
Abstract A few animals have been suspected to be intermediate hosts of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, a large-scale single-cell screening of SARS-CoV-2 target cells on a wide variety of animals is missing. Here, we constructed the single-cell atlas for 11 representative species in pets, livestock, poultry, and wildlife. Notably, the proportion of SARS-CoV-2 target cells in cat was found considerably higher than other species we investigated and SARS-CoV-2 target cells were detected in multiple cell types of domestic pig, implying the necessity to carefully evaluate the risk of cats during the current COVID-19 pandemic and keep pigs under surveillance for the possibility of becoming intermediate hosts in future coronavirus outbreak. Furthermore, we screened the expression patterns of receptors for 144 viruses, resulting in a comprehensive atlas of virus target cells. Taken together, our work provides a novel and fundamental strategy to screen virus target cells and susceptible species, based on single-cell transcriptomes we generated for domesticated animals and wildlife, which could function as a valuable resource for controlling current pandemics and serve as an early warning system for coping with future infectious disease threats..
Medienart: |
Preprint |
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Erscheinungsjahr: |
2020 |
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Erschienen: |
2020 |
Enthalten in: |
bioRxiv.org - (2020) vom: 17. Juni Zur Gesamtaufnahme - year:2020 |
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Sprache: |
Englisch |
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Beteiligte Personen: |
Chen, Dongsheng [VerfasserIn] |
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Links: |
Volltext [kostenfrei] |
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doi: |
10.1101/2020.06.13.149690 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
XBI018157130 |
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520 | |a Abstract A few animals have been suspected to be intermediate hosts of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, a large-scale single-cell screening of SARS-CoV-2 target cells on a wide variety of animals is missing. Here, we constructed the single-cell atlas for 11 representative species in pets, livestock, poultry, and wildlife. Notably, the proportion of SARS-CoV-2 target cells in cat was found considerably higher than other species we investigated and SARS-CoV-2 target cells were detected in multiple cell types of domestic pig, implying the necessity to carefully evaluate the risk of cats during the current COVID-19 pandemic and keep pigs under surveillance for the possibility of becoming intermediate hosts in future coronavirus outbreak. Furthermore, we screened the expression patterns of receptors for 144 viruses, resulting in a comprehensive atlas of virus target cells. Taken together, our work provides a novel and fundamental strategy to screen virus target cells and susceptible species, based on single-cell transcriptomes we generated for domesticated animals and wildlife, which could function as a valuable resource for controlling current pandemics and serve as an early warning system for coping with future infectious disease threats. | ||
700 | 1 | |a Sun, Jian |e verfasserin |4 aut | |
700 | 1 | |a Zhu, Jiacheng |e verfasserin |4 aut | |
700 | 1 | |a Ding, Xiangning |e verfasserin |4 aut | |
700 | 1 | |a Lan, Tianming |e verfasserin |4 aut | |
700 | 1 | |a Zhu, Linnan |e verfasserin |4 aut | |
700 | 1 | |a Xiang, Rong |e verfasserin |4 aut | |
700 | 1 | |a Ding, Peiwen |e verfasserin |4 aut | |
700 | 1 | |a Wang, Haoyu |e verfasserin |4 aut | |
700 | 1 | |a Wang, Xiaoling |e verfasserin |4 aut | |
700 | 1 | |a Wu, Weiying |e verfasserin |4 aut | |
700 | 1 | |a Qiu, Jiaying |e verfasserin |4 aut | |
700 | 1 | |a Wang, Shiyou |e verfasserin |4 aut | |
700 | 1 | |a Li, Haimeng |e verfasserin |4 aut | |
700 | 1 | |a An, Fuyu |e verfasserin |4 aut | |
700 | 1 | |a Bao, Heng |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Le |e verfasserin |4 aut | |
700 | 1 | |a Han, Lei |e verfasserin |4 aut | |
700 | 1 | |a Zhu, Yixin |e verfasserin |4 aut | |
700 | 1 | |a Wang, Xiran |e verfasserin |4 aut | |
700 | 1 | |a Wang, Feiyue |e verfasserin |4 aut | |
700 | 1 | |a Yuan, Yuting |e verfasserin |4 aut | |
700 | 1 | |a Wu, Wendi |e verfasserin |4 aut | |
700 | 1 | |a Sun, Chengcheng |e verfasserin |4 aut | |
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700 | 1 | |a Zeng, Yuying |e verfasserin |4 aut | |
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700 | 1 | |a Cui, ZeHua |e verfasserin |4 aut | |
700 | 1 | |a He, Qian |e verfasserin |4 aut | |
700 | 1 | |a Jiang, Sanjie |e verfasserin |4 aut | |
700 | 1 | |a Ma, Xiaoyan |e verfasserin |4 aut | |
700 | 1 | |a Feng, Weimin |e verfasserin |4 aut | |
700 | 1 | |a Xu, Yan |e verfasserin |4 aut | |
700 | 1 | |a Li, Fang |e verfasserin |4 aut | |
700 | 1 | |a Liu, Zhongmin |e verfasserin |4 aut | |
700 | 1 | |a Chen, Lei |e verfasserin |4 aut | |
700 | 1 | |a Chen, Fang |e verfasserin |4 aut | |
700 | 1 | |a Jin, Xin |e verfasserin |4 aut | |
700 | 1 | |a Qiu, Wei |e verfasserin |4 aut | |
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700 | 1 | |a Xu, Xun |e verfasserin |4 aut | |
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