Impaired T cell functions along with elevated activated Tregs at the early stage of asymptomatic SARS-CoV-2 infection
Summary Background Limited data are available on the T cell responses for the asymptomatic SARS-CoV-2 infection case.Methods An imported SARS-CoV-2 infection case in Wuhan was admitted in hospital for quarantine and observation. The T cell responses were followed up by flow cytometry analysis of the peripheral blood nonnuclear cells (PBMCs) at days 7, 13, 22, and 28 after admission.Findings We found the imported SARS-CoV-2 infection in Wuhan is an asymptomatic case. His T cell differentiation, proliferation and activation matched the classical kinetics of T cell responses induced by viral infection, but the activation maintained at a relatively low level. Function analysis indicated frequencies of IFN-γ producing CD4+ and CD8+ T cells were notably lower than that of the healthy controls (HC) at day 7, and then rebound gradually. But IFN-γ+CD8+ T cells were detained at a significant lower level even at day 28, when the SARS-CoV-2 virus had already become undetectable for 3 weeks. Moreover, percentage of IL-17 producing CD4+ T cells was also detained constantly at a much lower level compared to HC. At day 7, although percentage of Tregs was in normal range, the frequency of activated Treg (aTreg) was remarkably as high as 4·4-fold of that in HC.Interpretation The T cell activation in the asymptomatic SARS-CoV-2 infection experienced a significant suppression and presented impairment of Th1/Th17 and CD8+ T cell functions. Early elevation of the aTregs might play role in the activation and function of T cells in the asymptomatic SARS-CoV-2 infection..
| Medienart: |
|
|---|
Preprint| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
bioRxiv.org - (2020) vom: 23. Dez. Zur Gesamtaufnahme - year:2020 |
|---|
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Yang, Jingyi [VerfasserIn] |
|---|
| Links: |
Volltext [kostenfrei] |
|---|
| doi: |
10.1101/2020.05.25.20108852 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
XBI018001122 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | XBI018001122 | ||
| 003 | DE-627 | ||
| 005 | 20230429095554.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 200601s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1101/2020.05.25.20108852 |2 doi | |
| 035 | |a (DE-627)XBI018001122 | ||
| 035 | |a (DE-599)biorXiv10.1101/2020.05.25.20108852 | ||
| 035 | |a (biorXiv)10.1101/2020.05.25.20108852 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | |a 570 |q DE-84 | |
| 100 | 1 | |a Yang, Jingyi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Impaired T cell functions along with elevated activated Tregs at the early stage of asymptomatic SARS-CoV-2 infection |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a Computermedien |b c |2 rdamedia | ||
| 338 | |a Online-Ressource |b cr |2 rdacarrier | ||
| 520 | |a Summary Background Limited data are available on the T cell responses for the asymptomatic SARS-CoV-2 infection case.Methods An imported SARS-CoV-2 infection case in Wuhan was admitted in hospital for quarantine and observation. The T cell responses were followed up by flow cytometry analysis of the peripheral blood nonnuclear cells (PBMCs) at days 7, 13, 22, and 28 after admission.Findings We found the imported SARS-CoV-2 infection in Wuhan is an asymptomatic case. His T cell differentiation, proliferation and activation matched the classical kinetics of T cell responses induced by viral infection, but the activation maintained at a relatively low level. Function analysis indicated frequencies of IFN-γ producing CD4+ and CD8+ T cells were notably lower than that of the healthy controls (HC) at day 7, and then rebound gradually. But IFN-γ+CD8+ T cells were detained at a significant lower level even at day 28, when the SARS-CoV-2 virus had already become undetectable for 3 weeks. Moreover, percentage of IL-17 producing CD4+ T cells was also detained constantly at a much lower level compared to HC. At day 7, although percentage of Tregs was in normal range, the frequency of activated Treg (aTreg) was remarkably as high as 4·4-fold of that in HC.Interpretation The T cell activation in the asymptomatic SARS-CoV-2 infection experienced a significant suppression and presented impairment of Th1/Th17 and CD8+ T cell functions. Early elevation of the aTregs might play role in the activation and function of T cells in the asymptomatic SARS-CoV-2 infection. | ||
| 700 | 1 | |a Zhang, Ejuan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong, Maohua |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Qingyu |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Ke |e verfasserin |4 aut | |
| 700 | 1 | |a Shu, Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Dihan |e verfasserin |4 aut | |
| 700 | 1 | |a Xiang, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Jianbo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Xi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Dingyu |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Chaolin |e verfasserin |4 aut | |
| 700 | 1 | |a Shang, You |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Huimin |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t bioRxiv.org |g (2020) vom: 23. Dez. |
| 773 | 1 | 8 | |g year:2020 |g day:23 |g month:12 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1101/2020.05.25.20108852 |z kostenfrei |3 Volltext |
| 912 | |a GBV_XBI | ||
| 912 | |a SSG-OLC-PHA | ||
| 951 | |a AR | ||
| 952 | |j 2020 |b 23 |c 12 | ||
| 953 | |2 045F |a 570 | ||