Control of ribosomal protein synthesis by Microprocessor complex
Abstract Ribosome biogenesis in eukaryotes requires stoichiometric production and assembly of 80 ribosomal proteins (RPs) and 4 ribosomal RNAs, and its rate must be coordinated with cellular growth. The indispensable regulator of RP biosynthesis is the 5’-terminal oligopyrimidine (TOP) motif, spanning the transcription start site of all RP genes. Here we show that the Microprocessor complex, previously linked to the first step of processing microRNAs (miRNAs), coregulates RP expression by binding the TOP motif of nascent RP mRNAs and stimulating transcription elongation via resolution of DNA/RNA hybrids. Cell growth arrest triggers nuclear export and degradation of the Microprocessor protein Drosha by the E3 ubiquitin ligase Nedd4, accumulation of DNA/RNA hybrids at RP gene loci, decreased RP synthesis, and ribosome deficiency, hence synchronizing ribosome production with cell growth. Conditional deletion ofDroshain erythroid progenitors phenocopies human ribosomopathies, in which ribosomal insufficiency leads to anemia. Outlining a miRNA-independent role of the Microprocessor complex at the interphase between cell growth and ribosome biogenesis offers a new paradigm by which cells alter their protein biosynthetic capacity and cellular metabolism..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 22. Okt. Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jiang, Xuan [VerfasserIn] |
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| doi: |
10.1101/2020.04.24.060236 |
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| PPN (Katalog-ID): |
XBI017747147 |
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| 520 | |a Abstract Ribosome biogenesis in eukaryotes requires stoichiometric production and assembly of 80 ribosomal proteins (RPs) and 4 ribosomal RNAs, and its rate must be coordinated with cellular growth. The indispensable regulator of RP biosynthesis is the 5’-terminal oligopyrimidine (TOP) motif, spanning the transcription start site of all RP genes. Here we show that the Microprocessor complex, previously linked to the first step of processing microRNAs (miRNAs), coregulates RP expression by binding the TOP motif of nascent RP mRNAs and stimulating transcription elongation via resolution of DNA/RNA hybrids. Cell growth arrest triggers nuclear export and degradation of the Microprocessor protein Drosha by the E3 ubiquitin ligase Nedd4, accumulation of DNA/RNA hybrids at RP gene loci, decreased RP synthesis, and ribosome deficiency, hence synchronizing ribosome production with cell growth. Conditional deletion ofDroshain erythroid progenitors phenocopies human ribosomopathies, in which ribosomal insufficiency leads to anemia. Outlining a miRNA-independent role of the Microprocessor complex at the interphase between cell growth and ribosome biogenesis offers a new paradigm by which cells alter their protein biosynthetic capacity and cellular metabolism. | ||
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| 700 | 1 | |a Prabhakar, Amit |e verfasserin |4 aut | |
| 700 | 1 | |a Van der Voorn, Stephanie M. |e verfasserin |4 aut | |
| 700 | 1 | |a Ghatpande, Prajakta |e verfasserin |4 aut | |
| 700 | 1 | |a Celona, Barbara |e verfasserin |4 aut | |
| 700 | 1 | |a Venkataramanan, Srivats |e verfasserin |4 aut | |
| 700 | 1 | |a Calviello, Lorenzo |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Chuwen |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Wanpeng |e verfasserin |4 aut | |
| 700 | 1 | |a Black, Brian L. |e verfasserin |4 aut | |
| 700 | 1 | |a Floor, Stephen N. |e verfasserin |4 aut | |
| 700 | 1 | |a Lagna, Giorgio |e verfasserin |4 aut | |
| 700 | 1 | |a Hata, Akiko |e verfasserin |4 aut | |
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