Capture and visualization of live Mycobacterium tuberculosis bacilli from tuberculosis bioaerosols
Abstract The tuberculosis (TB) pandemic demands urgent interventions such as those designed to interrupt Mycobacterium tuberculosis (Mtb) transmission, a challenge exacerbated by our poor understanding of the events enabling successful transfer of infectious bacilli between hosts. To address this problem, we developed the Respiratory Aerosol Sampling Chamber (RASC), a personal clean-room equipped with high-efficiency filtration and sampling technologies that allow biosafe capture and isolation of particulate matter – including Mtb bacilli – released by patients during natural breathing and (non-induced) cough. Here, we demonstrate the use of DMN-trehalose labelling to detect and quantify live Mtb bacilli among complex bioaerosol samples arrayed in a bespoke nanowell device following capture in the RASC. A pilot study identified Mtb in more than 85 % of known TB patients, improving significantly on previous work which has relied on animal infection and cough sampling to estimate transmission events. Moreover, intra-patient comparisons of bioaerosol and sputum samples indicated that Mtb aerosols likely derive from a compartment other than sputum. These results support the utility of the RASC platform for research aimed at interrupting Mtb transmission, including the non-invasive detection of Mtb-infected individuals who are predicted to contribute to bacillary spread despite the absence of clinical symptoms..
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Preprint| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
bioRxiv.org - (2021) vom: 15. Dez. Zur Gesamtaufnahme - year:2021 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dinkele, Ryan [VerfasserIn] |
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| Links: |
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| doi: |
10.1101/2019.12.23.887729 |
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| PPN (Katalog-ID): |
XBI000688002 |
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| 520 | |a Abstract The tuberculosis (TB) pandemic demands urgent interventions such as those designed to interrupt Mycobacterium tuberculosis (Mtb) transmission, a challenge exacerbated by our poor understanding of the events enabling successful transfer of infectious bacilli between hosts. To address this problem, we developed the Respiratory Aerosol Sampling Chamber (RASC), a personal clean-room equipped with high-efficiency filtration and sampling technologies that allow biosafe capture and isolation of particulate matter – including Mtb bacilli – released by patients during natural breathing and (non-induced) cough. Here, we demonstrate the use of DMN-trehalose labelling to detect and quantify live Mtb bacilli among complex bioaerosol samples arrayed in a bespoke nanowell device following capture in the RASC. A pilot study identified Mtb in more than 85 % of known TB patients, improving significantly on previous work which has relied on animal infection and cough sampling to estimate transmission events. Moreover, intra-patient comparisons of bioaerosol and sputum samples indicated that Mtb aerosols likely derive from a compartment other than sputum. These results support the utility of the RASC platform for research aimed at interrupting Mtb transmission, including the non-invasive detection of Mtb-infected individuals who are predicted to contribute to bacillary spread despite the absence of clinical symptoms. | ||
| 700 | 1 | |a Gessner, Sophia |e verfasserin |4 aut | |
| 700 | 1 | |a Koch, Anastasia S. |e verfasserin |4 aut | |
| 700 | 1 | |a Morrow, Carl |e verfasserin |4 aut | |
| 700 | 1 | |a Gqada, Melitta |e verfasserin |4 aut | |
| 700 | 1 | |a Kamariza, Mireille |e verfasserin |4 aut | |
| 700 | 1 | |a Bertozzi, Carolyn R. |e verfasserin |4 aut | |
| 700 | 1 | |a Smith, Brian |e verfasserin |4 aut | |
| 700 | 1 | |a McLoud, Courtney |e verfasserin |4 aut | |
| 700 | 1 | |a Kamholz, Andrew |e verfasserin |4 aut | |
| 700 | 1 | |a Bryden, Wayne |e verfasserin |4 aut | |
| 700 | 1 | |a Call, Charles |e verfasserin |4 aut | |
| 700 | 1 | |a Mizrahi, Valerie |e verfasserin |4 aut | |
| 700 | 1 | |a Wood, Robin |e verfasserin |4 aut | |
| 700 | 1 | |a Warner, Digby F. |e verfasserin |4 aut | |
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