Zebrafish<i>dazl</i>regulates cystogenesis upstream of the meiotic transition and germline stem cell specification and independent of meiotic checkpoints
Abstract Fertility and gamete reserves are maintained by asymmetric divisions of the germline stem cells to produce new stem cells or daughters that differentiate as gametes. Before entering meiosis, differentiating germ cells (GCs) of sexual animals typically undergo cystogenesis. This evolutionary conserved process involves synchronous and incomplete mitotic divisions of a germ cell daughter (cystoblast) to generate sister cells connected by stable intercellular bridges that facilitate exchange of materials to support a large synchronous population of gamete progenitors. Here we investigate cystogenesis in zebrafish and identified Deleted in azoospermia (Dazl), a conserved vertebrate RNA binding protein as a regulator of this process. Analysis ofdazlmutants revealed an essential role for Dazl in regulating incomplete cytokinesis and germline cyst formation before the meiotic transition. Accordingly,dazlmutant GCs form defective ring canals, and ultimately remain as individual cells that fail to differentiate as meiocytes. In addition to promoting cystoblast divisions and meiotic entry,dazlfunction is required upstream of germline stem cell establishment and fertility.Summary Statement We show that zebrafishdazlis required for incomplete cytokinesis to generate germline cysts during cystogenesis, acts upstream of germline stem cell establishment, and is required for meiosis, and fertility..
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Preprint| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
bioRxiv.org - (2022) vom: 09. Okt. Zur Gesamtaufnahme - year:2022 |
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| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bertho, Sylvain [VerfasserIn] |
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| Links: |
Volltext [kostenfrei] |
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| doi: |
10.1101/2019.12.23.887687 |
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| PPN (Katalog-ID): |
XBI000687979 |
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| 100 | 1 | |a Bertho, Sylvain |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Zebrafish<i>dazl</i>regulates cystogenesis upstream of the meiotic transition and germline stem cell specification and independent of meiotic checkpoints |
| 264 | 1 | |c 2022 | |
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| 520 | |a Abstract Fertility and gamete reserves are maintained by asymmetric divisions of the germline stem cells to produce new stem cells or daughters that differentiate as gametes. Before entering meiosis, differentiating germ cells (GCs) of sexual animals typically undergo cystogenesis. This evolutionary conserved process involves synchronous and incomplete mitotic divisions of a germ cell daughter (cystoblast) to generate sister cells connected by stable intercellular bridges that facilitate exchange of materials to support a large synchronous population of gamete progenitors. Here we investigate cystogenesis in zebrafish and identified Deleted in azoospermia (Dazl), a conserved vertebrate RNA binding protein as a regulator of this process. Analysis ofdazlmutants revealed an essential role for Dazl in regulating incomplete cytokinesis and germline cyst formation before the meiotic transition. Accordingly,dazlmutant GCs form defective ring canals, and ultimately remain as individual cells that fail to differentiate as meiocytes. In addition to promoting cystoblast divisions and meiotic entry,dazlfunction is required upstream of germline stem cell establishment and fertility.Summary Statement We show that zebrafishdazlis required for incomplete cytokinesis to generate germline cysts during cystogenesis, acts upstream of germline stem cell establishment, and is required for meiosis, and fertility. | ||
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| 700 | 1 | |a Banisch, Torsten U. |e verfasserin |4 aut | |
| 700 | 1 | |a Bandemer, Jan |e verfasserin |4 aut | |
| 700 | 1 | |a Raz, Erez |e verfasserin |4 aut | |
| 700 | 1 | |a Marlow, Florence L. |e verfasserin |4 aut | |
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