Fluorescent Visualization of the Distribution of $ GFP^{+} $ Donor Cells in Mouse Organs after Transplantation of Native or Cryopreserved Bone Marrow

Abstract— Cryopreservation is the only way to preserve native bone marrow (BM) stem cells for application in regenerative medicine or before ablative therapy of malignant diseases. Transplantation of autologous BM stem cells (autotransplantation) has not been sufficiently studied in terms of the interaction of transplanted cells with the host (recipient) organism. The aim of this work was to evaluate the effectiveness of syngeneic transplantation as a model of autotransplantation, of native or cryopreserved BM to sublethally irradiated recipient mice. Mice carrying the green fluorescent protein (GFP) gene bred on the basis of C57BL/6 inbred strain served as donors. C57BL/6 mice were used as recipients. Using fluorescence microscopy, we studied the level of chimerism in organs of the lymphomyeloid complex of the blood system (bone marrow, thymus, spleen, blood, and colon) in recipient mice at different terms after transplantation of native or cryopreserved $ GFP^{+} $ cells of donor’s whole syngeneic BM. Differences in the ability of $ GFP^{+} $ cells to engraft the body were assessed on the basis of their amount (percentage) in the cell suspension of recipient BM, spleen, and thymus. $ GFP^{+} $ cells in recipients that were transplanted with thawed BM appeared in the examined organs 7–8 days later than in recipients after transplantation of native BM. However, already 14–21 days after transplantation, the relative number of donor cells in the recipient organs of both groups did not differ significantly. In whole organs (BM, spleen, thymus, and colon), or their cross-sections chimerism after transplantation was detected 2–3 days earlier than in a suspension of these organs. It was also shown that the donor BM mesenchymal cells were involved in the stroma recovery of all organs damaged by radiation, as evidenced by the presence of fibroblast-like $ GFP^{+} $ cells, which was especially evident in the thymus of recipients of both groups. Thus, our investigation has shown that BM cells cryopreserved according to the method applied are sufficiently viable for effective histogenesis of the studied organs of the blood system..

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:16

Enthalten in:

Cell and tissue biology - 16(2022), 2 vom: Apr., Seite 167-177

Sprache:

Englisch

Beteiligte Personen:

Sergievich, L. A. [VerfasserIn]
Bogdanenko, E. V. [VerfasserIn]
Karnaukhov, A. V. [VerfasserIn]
Karnaukhova, N. A. [VerfasserIn]
Lizunova, I. A. [VerfasserIn]

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Anmerkungen:

© Pleiades Publishing, Ltd. 2022. ISSN 1990-519X, Cell and Tissue Biology, 2022, Vol. 16, No. 2, pp. 167–177. © Pleiades Publishing, Ltd., 2022. Russian Text © The Author(s), 2021, published in Tsitologiya, 2021, Vol. 63, No. 6, pp. 568–579.

doi:

10.1134/S1990519X22020092

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

SPR050646222