In Vitro Propagation of Artemisia japonica
An effective protocol for in vitro propagation was developed for Artemisia japonica using nodal explants. The efficiency of the explants to induce the multiple shoots on Murashige and Skoog (MS) medium supplemented with 2.5, 5.0, 7.5, and 10 µM of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 1-napthalene acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), 6-benzylaminopurine (BAP), thidiazuron (TDZ), kinetin (Kn), and 2-isopentenyl adenine (2iP) was tested. A maximum of 20 axillary shoots per explant were induced on MS medium supplemented with 10 µM BAP. Shoots were rooted on MS medium supplemented with IBA (10 µM). The resulting plantlets were hardened in a mixture of cocopeat and perlite (1:1). Cytological analysis revealed that regenerated plants had 2n = 18 chromosomes, which was same as that of the mother plant..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2017 |
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| Erschienen: |
2017 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
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| Enthalten in: |
Journal of herbs, spices & medicinal plants - 23(2017), 1, Seite 36-43 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shinde, Smita [VerfasserIn] |
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| doi: |
10.1080/10496475.2016.1256010 |
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| 520 | |a An effective protocol for in vitro propagation was developed for Artemisia japonica using nodal explants. The efficiency of the explants to induce the multiple shoots on Murashige and Skoog (MS) medium supplemented with 2.5, 5.0, 7.5, and 10 µM of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 1-napthalene acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), 6-benzylaminopurine (BAP), thidiazuron (TDZ), kinetin (Kn), and 2-isopentenyl adenine (2iP) was tested. A maximum of 20 axillary shoots per explant were induced on MS medium supplemented with 10 µM BAP. Shoots were rooted on MS medium supplemented with IBA (10 µM). The resulting plantlets were hardened in a mixture of cocopeat and perlite (1:1). Cytological analysis revealed that regenerated plants had 2n = 18 chromosomes, which was same as that of the mother plant. | ||
| 540 | |a Nutzungsrecht: © 2017 Taylor & Francis Group, LLC 2017 | ||
| 650 | 4 | |a micropropagation | |
| 650 | 4 | |a axillary shoots | |
| 650 | 4 | |a Acclimatization | |
| 700 | 1 | |a Katewal, Pooja R |4 oth | |
| 700 | 1 | |a Shanbhag, Divya D |4 oth | |
| 700 | 1 | |a Joseph, Kadanthottu Sebastian |4 oth | |
| 700 | 1 | |a Murthy, H. N |4 oth | |
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