miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8
This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a. miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3'UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively. The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients. IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels. The luciferase activity assays indicated that IL-8 is a target of miR-106a. Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects. This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8..
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2015 |
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| Erschienen: |
2015 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2015 |
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| Enthalten in: |
Mediators of inflammation - 2015(2015) |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hong, Zhongsi [VerfasserIn] |
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| Links: |
Volltext |
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| doi: |
10.1155/2015/629862 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
OLC1959349503 |
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| 245 | 1 | 0 | |a miR-106a Is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B and Associated with Enhanced Levels of Interleukin-8 |
| 264 | 1 | |c 2015 | |
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| 520 | |a This study aimed to investigate miR-106a expression in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B (CHB) patients and to analyze the function of miR-106a. miR-106a expression levels in PBMCs from 40 healthy controls and 56 CHB patients were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). The luciferase activity assays were used to determine whether miR-106a binds to 3'UTR of IL-8. miR-106a mimics and inhibitors were transfected into healthy PBMCs. IL-8 mRNA and protein levels were detected and determined by qRT-PCR and ELISA, respectively. The qRT-PCR results suggested that the PBMC miR-106a levels were decreased in CHB patients. IL-8 was augmented in CHB patients and was inversely correlated with miR-106a levels. The luciferase activity assays indicated that IL-8 is a target of miR-106a. Exogenous expression of miR-106a could significantly repress IL-8 expression at both mRNA and protein levels in PBMCs, whereas miR-106a inhibitor had the opposite effects. This study suggested that miR-106a is downregulated in PBMCs of CHB patients and that miR-106a may play an important role in CHB by targeting IL-8. | ||
| 540 | |a Nutzungsrecht: Copyright © 2015 Zhongsi Hong et al. 2015 | ||
| 650 | 4 | |a Chemokines | |
| 650 | 4 | |a Liver cancer | |
| 650 | 4 | |a Viral infections | |
| 650 | 4 | |a Deoxyribonucleic acid--DNA | |
| 650 | 4 | |a Studies | |
| 650 | 4 | |a Epigenetics | |
| 650 | 4 | |a Medical prognosis | |
| 650 | 4 | |a Pathogenesis | |
| 650 | 4 | |a Cytomegalovirus | |
| 650 | 4 | |a Inflammation | |
| 650 | 4 | |a Hepatitis | |
| 650 | 4 | |a Gene expression | |
| 650 | 4 | |a Health care | |
| 650 | 4 | |a Infectious diseases | |
| 650 | 4 | |a DNA methylation | |
| 650 | 4 | |a Cytokines | |
| 650 | 4 | |a Proteins | |
| 650 | 4 | |a Infections | |
| 650 | 4 | |a Hospitals | |
| 650 | 4 | |a RB1-214 | |
| 650 | 4 | |a Pathology | |
| 650 | 4 | |a Medicine | |
| 700 | 1 | |a Hong, Haiyu |4 oth | |
| 700 | 1 | |a Liu, Jian |4 oth | |
| 700 | 1 | |a Zheng, Xiaobin |4 oth | |
| 700 | 1 | |a Huang, Mingxing |4 oth | |
| 700 | 1 | |a Li, Chunna |4 oth | |
| 700 | 1 | |a Xia, Jinyu |4 oth | |
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| 856 | 4 | 1 | |u http://dx.doi.org/10.1155/2015/629862 |3 Volltext |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/26265888 |
| 856 | 4 | 2 | |u http://search.proquest.com/docview/1709456730 |
| 856 | 4 | 2 | |u http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=4525765&tool=pmcentrez&rendertype=abstract |
| 856 | 4 | 2 | |u https://doaj.org/article/8894a28b6c1e4bdc87dd4590c7fef27e |
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| 952 | |d 2015 |j 2015 | ||