Investigation of the structural changes in the hippocampus and prefrontal cortex using FTIR spectroscopy in sleep deprived mice
Copyright © 2024 Elsevier B.V. All rights reserved..
Sleep is a basic, physiological requirement for living things to survive and is a process that covers one third of our lives. Melatonin is a hormone that plays an important role in the regulation of sleep. Sleep deprivation affect brain structures and functions. Sleep deprivation causes a decrease in brain activity, with particularly negative effects on the hippocampus and prefrontal cortex. Despite the essential role of protein and lipids vibrations, polysaccharides, fatty acid side chains functional groups, and ratios between amides in brain structures and functions, the brain chemical profile exposed to gentle handling sleep deprivation model versus Melatonin exposure remains unexplored. Therefore, the present study, aims to investigate a molecular profile of these regions using FTIR spectroscopy measurement's analysis based on lipidomic approach with chemometrics and multivariate analysis to evaluate changes in lipid composition in the hippocampus, prefrontal regions of the brain. In this study, C57BL/6J mice were randomly assigned to either the control or sleep deprivation group, resulting in four experimental groups: Control (C) (n = 6), Control + Melatonin (C + M) (n = 6), Sleep Deprivation (S) (n = 6), and Sleep Deprivation + Melatonin (S + M) (n = 6). Interventions were administered each morning via intraperitoneal injections of melatonin (10 mg/kg) or vehicle solution (%1 ethanol + saline), while the S and S + M groups underwent 6 h of daily sleep deprivation from using the Gentle Handling method. All mice were individually housed in cages with ad libitum access to food and water within a 12-hour light-dark cycle. Results presented that the brain regions affected by insomnia. The structure of phospholipids, changed. Yet, not only changes in lipids but also in amides were noticed in hippocampus and prefrontal cortex tissues. Additionally, FTIR results showed that melatonin affected the lipids as well as the amides fraction in cortex and hippocampus collected from both control and sleep deprivation groups.
Media Type: |
Electronic Article |
---|
Year of Publication: |
2024 |
---|---|
Publication: |
2024 |
Contained In: |
To Main Record - volume:321 |
---|---|
Contained In: |
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy - 321(2024) vom: 15. Aug., Seite 124702 |
Language: |
English |
---|
Contributors: |
Saribal, Devrim [Author] |
---|
Links: |
---|
Notes: |
Date Completed 10.08.2024 Date Revised 10.08.2024 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1016/j.saa.2024.124702 |
---|
funding: |
|
---|---|
Supporting institution / Project title: |
|
PPN (Catalogue-ID): |
NLM374056382 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM374056382 | ||
003 | DE-627 | ||
005 | 20240811232133.0 | ||
007 | cr uuu---uuuuu | ||
008 | 240626s2024 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.saa.2024.124702 |2 doi | |
028 | 5 | 2 | |a pubmed24n1498.xml |
035 | |a (DE-627)NLM374056382 | ||
035 | |a (NLM)38917751 | ||
035 | |a (PII)S1386-1425(24)00868-0 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Saribal, Devrim |e verfasserin |4 aut | |
245 | 1 | 0 | |a Investigation of the structural changes in the hippocampus and prefrontal cortex using FTIR spectroscopy in sleep deprived mice |
264 | 1 | |c 2024 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 10.08.2024 | ||
500 | |a Date Revised 10.08.2024 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2024 Elsevier B.V. All rights reserved. | ||
520 | |a Sleep is a basic, physiological requirement for living things to survive and is a process that covers one third of our lives. Melatonin is a hormone that plays an important role in the regulation of sleep. Sleep deprivation affect brain structures and functions. Sleep deprivation causes a decrease in brain activity, with particularly negative effects on the hippocampus and prefrontal cortex. Despite the essential role of protein and lipids vibrations, polysaccharides, fatty acid side chains functional groups, and ratios between amides in brain structures and functions, the brain chemical profile exposed to gentle handling sleep deprivation model versus Melatonin exposure remains unexplored. Therefore, the present study, aims to investigate a molecular profile of these regions using FTIR spectroscopy measurement's analysis based on lipidomic approach with chemometrics and multivariate analysis to evaluate changes in lipid composition in the hippocampus, prefrontal regions of the brain. In this study, C57BL/6J mice were randomly assigned to either the control or sleep deprivation group, resulting in four experimental groups: Control (C) (n = 6), Control + Melatonin (C + M) (n = 6), Sleep Deprivation (S) (n = 6), and Sleep Deprivation + Melatonin (S + M) (n = 6). Interventions were administered each morning via intraperitoneal injections of melatonin (10 mg/kg) or vehicle solution (%1 ethanol + saline), while the S and S + M groups underwent 6 h of daily sleep deprivation from using the Gentle Handling method. All mice were individually housed in cages with ad libitum access to food and water within a 12-hour light-dark cycle. Results presented that the brain regions affected by insomnia. The structure of phospholipids, changed. Yet, not only changes in lipids but also in amides were noticed in hippocampus and prefrontal cortex tissues. Additionally, FTIR results showed that melatonin affected the lipids as well as the amides fraction in cortex and hippocampus collected from both control and sleep deprivation groups | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Bayesian optimization | |
650 | 4 | |a FTIR | |
650 | 4 | |a Hippocampus | |
650 | 4 | |a Prefrontal cortex | |
650 | 4 | |a Principal Component Analysis (PCA) | |
650 | 4 | |a Receiver Operating Characteristic (ROC) | |
650 | 4 | |a Sleep deprivation | |
650 | 4 | |a Support Vector Machine (SVM) | |
650 | 7 | |a Melatonin |2 NLM | |
650 | 7 | |a JL5DK93RCL |2 NLM | |
650 | 7 | |a Lipids |2 NLM | |
700 | 1 | |a Çalis, Hakan |e verfasserin |4 aut | |
700 | 1 | |a Ceylan, Zeynep |e verfasserin |4 aut | |
700 | 1 | |a Depciuch, Joanna |e verfasserin |4 aut | |
700 | 1 | |a Cebulski, Jozef |e verfasserin |4 aut | |
700 | 1 | |a Guleken, Zozan |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy |d 1998 |g 321(2024) vom: 15. Aug., Seite 124702 |w (DE-627)NLM090551192 |x 1873-3557 |7 nnns |
773 | 1 | 8 | |g volume:321 |g year:2024 |g day:15 |g month:08 |g pages:124702 |
856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.saa.2024.124702 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 321 |j 2024 |b 15 |c 08 |h 124702 |