Quantitative or qualitative biomolecular changes in blood serum composition induced by childhood obesity : A Fourier transform infrared examination

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Childhood obesity (CO) negatively affects one in three children and stands as the fourth most common risk factor of health and well-being. Clarifying the molecular and structural modifications that transpire during the development of obesity is crucial for understanding its progression and devising effective therapies. The study was indeed conducted as part of an ongoing CO treatment trial, where data were collected from children diagnosed with CO before the initiation of non-drug treatment interventions. Our primary aim was to analyze the biochemical changes associated with childhood obesity, specifically focusing on concentrations of lipids, lipoproteins, insulin, and glucose. By comparing these parameters between the CO group (n = 60) and a control group of healthy children (n = 43), we sought to elucidate the metabolic differences present in individuals with CO. Our biochemical analyses unveiled lower LDL (low-density lipoproteins) levels and higher HDL (high-density lipoproteins), cholesterol, triglycerides, insulin, and glucose levels in CO individuals compared to controls. To scrutinize these changes in more detail, we employed Fourier transform infrared (FTIR) spectroscopy on the serum samples. Our results indicated elevated levels of lipids and proteins in the serum of CO, compared to controls. Additionally, we noted structural changes in the vibrations of glucose, β-sheet, and lipids in CO group. The FTIR technique, coupled with principal component analysis (PCA), demonstrated a marked differentiation between CO and controls, particularly in the FTIR region corresponding to amide and lipids. The Pearson test revealed a stronger correlation between biochemical data and FTIR spectra than between 2nd derivative FTIR spectra. Overall, our study provides valuable insights into the molecular and structural changes occurring in CO.

Media Type:

Electronic Article

Year of Publication:

2024

Publication:

2024

Contained In:

To Main Record - volume:313

Contained In:

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy - 313(2024) vom: 15. Apr., Seite 124153

Language:

English

Contributors:

Guleken, Zozan [Author]
Ceylan, Zeynep [Author]
Çeçen, Serpil [Author]
Elgörmüş, Yusuf [Author]
Cebulski, Jozef [Author]
Depciuch, Joanna [Author]

Links:

Volltext

Keywords:

Bimolecular Structure
Chemometrics
Childhood obesity
FTIR spectroscopy
Glucose
IY9XDZ35W2
Insulin
Journal Article
Lipoproteins
Secondary Protein Structure
Serum profile

Notes:

Date Completed 02.04.2024

Date Revised 02.04.2024

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.saa.2024.124153

funding:

Supporting institution / Project title:

PPN (Catalogue-ID):

NLM369821408