A triple-aptamer tetrahedral DNA nanostructures based carbon-nanotube-array transistor biosensor for rapid virus detection
Copyright © 2023 Elsevier B.V. All rights reserved..
Outbreaks of infectious viruses cause enormous challenges to global public health. Recently, the coronavirus disease 2019 (COVID-19) induced by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has severely threatened human health and resulted in the global pandemic. A strategy to detect SARS-CoV-2 with both fast sensing speed and high accuracy is urgently required. Here, rapid detection of SARS-CoV-2 antigen using carbon-nanotube-array-based thin-film transistor (CNT-array-based TFT) biosensors merged with tetrahedral DNA nanostructures (TDNs) and triple aptamers is demonstrated for the first time. Compared with CNT-network-based TFT biosensors and metal-electrode-based CNT-TFT biosensors, the response of CNT-array-based TFT biosensors can be enhanced up to 102% for SARS-CoV-2 receptor-binding domain (RBD) detection, which is supported by its sensing mechanism. By combining TDNs with triple aptamers, the biosensor has realized the wildtype SARS-CoV-2 RBD detection in a broad detection range spanning eight orders of magnitude with a low limit of detection (LOD) of 10 aM (6 copies/μL) owing to the improved protein capture efficiency. Moreover, the triple-aptamer biosensor platform has achieved the detection of SARS-CoV-2 Omicron RBD in a low LOD of 6 aM (3.6 copies/μL). Additionally, the CNT-array-based TFT biosensors have exhibited excellent specificity, enabling identification among SARS-CoV-2 antigen, SARS-CoV antigen and MERS-CoV antigen. The platform of CNT-array-based TFT biosensors combined with TDNs and triple aptamers provides a high-performance and rapid approach for SARS-CoV-2 detection, and its versatility by altering specific aptamers enables the possibility for rapid virus detection.
| Media Type: |
Electronic Article |
|---|
| Year of Publication: |
2024 2023 |
|---|---|
| Publication: |
2024 |
| Contained In: |
To Main Record - volume:266 |
|---|---|
| Contained In: |
Talanta - 266(2023), Pt 1 vom: 01. Jan., Seite 124973 |
| Language: |
English |
|---|
| Contributors: |
Ma, Shenhui [Author] |
|---|
| Links: |
|---|
| Keywords: |
9007-49-2 |
|---|
| Notes: |
Date Completed 20.09.2023 Date Revised 20.09.2023 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.talanta.2023.124973 |
|---|
| funding: |
|
|---|---|
| Supporting institution / Project title: |
|
| PPN (Catalogue-ID): |
NLM360133819 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM360133819 | ||
| 003 | DE-627 | ||
| 005 | 20230920232616.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 230729s2024 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.talanta.2023.124973 |2 doi | |
| 028 | 5 | 2 | |a pubmed23n1479.xml |
| 035 | |a (DE-627)NLM360133819 | ||
| 035 | |a (NLM)37506519 | ||
| 035 | |a (PII)S0039-9140(23)00724-5 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ma, Shenhui |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A triple-aptamer tetrahedral DNA nanostructures based carbon-nanotube-array transistor biosensor for rapid virus detection |
| 264 | 1 | |c 2024 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 20.09.2023 | ||
| 500 | |a Date Revised 20.09.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2023 Elsevier B.V. All rights reserved. | ||
| 520 | |a Outbreaks of infectious viruses cause enormous challenges to global public health. Recently, the coronavirus disease 2019 (COVID-19) induced by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has severely threatened human health and resulted in the global pandemic. A strategy to detect SARS-CoV-2 with both fast sensing speed and high accuracy is urgently required. Here, rapid detection of SARS-CoV-2 antigen using carbon-nanotube-array-based thin-film transistor (CNT-array-based TFT) biosensors merged with tetrahedral DNA nanostructures (TDNs) and triple aptamers is demonstrated for the first time. Compared with CNT-network-based TFT biosensors and metal-electrode-based CNT-TFT biosensors, the response of CNT-array-based TFT biosensors can be enhanced up to 102% for SARS-CoV-2 receptor-binding domain (RBD) detection, which is supported by its sensing mechanism. By combining TDNs with triple aptamers, the biosensor has realized the wildtype SARS-CoV-2 RBD detection in a broad detection range spanning eight orders of magnitude with a low limit of detection (LOD) of 10 aM (6 copies/μL) owing to the improved protein capture efficiency. Moreover, the triple-aptamer biosensor platform has achieved the detection of SARS-CoV-2 Omicron RBD in a low LOD of 6 aM (3.6 copies/μL). Additionally, the CNT-array-based TFT biosensors have exhibited excellent specificity, enabling identification among SARS-CoV-2 antigen, SARS-CoV antigen and MERS-CoV antigen. The platform of CNT-array-based TFT biosensors combined with TDNs and triple aptamers provides a high-performance and rapid approach for SARS-CoV-2 detection, and its versatility by altering specific aptamers enables the possibility for rapid virus detection | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Carbon nanotube array | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 4 | |a Tetrahedral DNA nanostructure | |
| 650 | 4 | |a Thin-film transistors | |
| 650 | 4 | |a Triple aptamers | |
| 650 | 7 | |a Nanotubes, Carbon |2 NLM | |
| 650 | 7 | |a DNA |2 NLM | |
| 650 | 7 | |a 9007-49-2 |2 NLM | |
| 650 | 7 | |a Oligonucleotides |2 NLM | |
| 700 | 1 | |a Ren, Qinqi |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Leying |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Zhihong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Jiahao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yaping |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Min |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Talanta |d 1966 |g 266(2023), Pt 1 vom: 01. Jan., Seite 124973 |w (DE-627)NLM000143693 |x 1873-3573 |7 nnns |
| 773 | 1 | 8 | |g volume:266 |g year:2023 |g number:Pt 1 |g day:01 |g month:01 |g pages:124973 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.talanta.2023.124973 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 936 | u | w | |d 266 |j 2023 |e Pt 1 |b 01 |c 01 |h 124973 |
| 951 | |a AR | ||
| 952 | |d 266 |j 2023 |e Pt 1 |b 01 |c 01 |h 124973 | ||