Functional attachment of primary neurons and glia on radiopaque implantable biomaterials for nerve repair
Copyright © 2023 Elsevier Inc. All rights reserved..
Repairing peripheral nerve injuries remains a challenge, even with use of auxiliary implantable biomaterial conduits. After implantation the location or function of polymeric devices cannot be assessed via clinical imaging modalities. Adding nanoparticle contrast agents into polymers can introduce radiopacity enabling imaging using computed tomography. Radiopacity must be balanced with changes in material properties impacting device function. In this study radiopaque composites were made from polycaprolactone and poly(lactide-co-glycolide) 50:50 and 85:15 with 0-40 wt% tantalum oxide (TaOx) nanoparticles. To achieve radiopacity, ≥5 wt% TaOx was required, with ≥20 wt% TaOx reducing mechanical properties and causing nanoscale surface roughness. Composite films facilitated nerve regeneration in an in vitro co-culture of adult glia and neurons, measured by markers for myelination. The ability of radiopaque films to support regeneration was driven by the properties of the polymer, with 5-20 wt% TaOx balancing imaging functionality with biological response and proving that in situ monitoring is feasible.
| Errataetall: | |
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| Media Type: |
Electronic Article |
| Year of Publication: |
2023 |
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| Publication: |
2023 |
| Contained In: |
To Main Record - volume:52 |
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| Contained In: |
Nanomedicine : nanotechnology, biology, and medicine - 52(2023) vom: 15. Aug., Seite 102692 |
| Language: |
English |
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| Contributors: |
Pawelec, Kendell M [Author] |
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| Links: |
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| Keywords: |
Biocompatible Materials |
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| Notes: |
Date Completed 01.09.2023 Date Revised 01.09.2023 published: Print-Electronic UpdateOf: bioRxiv. 2023 Jan 06;:. - PMID 36711915 Citation Status MEDLINE |
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| doi: |
10.1016/j.nano.2023.102692 |
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| funding: |
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| 520 | |a Repairing peripheral nerve injuries remains a challenge, even with use of auxiliary implantable biomaterial conduits. After implantation the location or function of polymeric devices cannot be assessed via clinical imaging modalities. Adding nanoparticle contrast agents into polymers can introduce radiopacity enabling imaging using computed tomography. Radiopacity must be balanced with changes in material properties impacting device function. In this study radiopaque composites were made from polycaprolactone and poly(lactide-co-glycolide) 50:50 and 85:15 with 0-40 wt% tantalum oxide (TaOx) nanoparticles. To achieve radiopacity, ≥5 wt% TaOx was required, with ≥20 wt% TaOx reducing mechanical properties and causing nanoscale surface roughness. Composite films facilitated nerve regeneration in an in vitro co-culture of adult glia and neurons, measured by markers for myelination. The ability of radiopaque films to support regeneration was driven by the properties of the polymer, with 5-20 wt% TaOx balancing imaging functionality with biological response and proving that in situ monitoring is feasible | ||
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