Standardization of in-house anti-IgG and IgA ELISAs for the detection of COVID-19

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19). RT-PCR detection of viral RNA represents the gold standard method for diagnosis of COVID-19. However, multiple diagnostic tests are needed for acute disease diagnosis and assessing immunity during the COVID-19 outbreak. Here, we developed in-house anti-RBD IgG and IgA enzyme-linked immunosorbent assays (ELISAs) using a well-defined serum sample panel for screening and identification of human SARS-CoV-2 infection. We found that our in-house anti-SARS-CoV-2 IgG ELISA displayed a 93.5% sensitivity and 98.8% specificity whereas our in-house anti-SARS-CoV-2 IgA ELISA provided assay sensitivity and specificity at 89.5% and 99.4%, respectively. The agreement kappa values of our in-house anti-SARS-CoV-2 IgG and IgA ELISA assays were deemed to be excellent and fair, respectively, when compared to RT-PCR and excellent for both assays when compared to Euroimmun anti-SARS-CoV-2 IgG and IgA ELISAs. These data indicate that our in-house anti-SARS-CoV-2 IgG and IgA ELISAs are compatible performing assays for the detection of SARS-CoV-2 infection.

Media Type:

Electronic Article

Year of Publication:




Contained In:

To Main Record - volume:18

Contained In:

PloS one - 18(2023), 6 vom: 01., Seite e0287107




Rungrojcharoenkit, Kamonthip [Author]
Suthangkornkul, Rungarun [Author]
Utennam, Darunee [Author]
Buddhari, Darunee [Author]
Pinpaiboon, Soontorn [Author]
Mongkolsirichaikul, Duangrat [Author]
Fernandez, Stefan [Author]
Jones, Anthony R [Author]
Cotrone, Thomas S [Author]
Hunsawong, Taweewun [Author]




Antibodies, Viral
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.


Date Completed 12.06.2023

Date Revised 20.11.2023

published: Electronic-eCollection

Citation Status MEDLINE




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