Deep mining and quantification of oxidized cholesteryl esters discovers potential biomarkers involved in breast cancer by liquid chromatography-mass spectrometry

Copyright © 2021 Elsevier B.V. All rights reserved..

Oxidized cholesteryl ester (OxCE) is produced by the oxidation of cholesteryl ester (CE) in the cores of lipoproteins. OxCE production and oxidative stress have been largely associated with breast cancer. Herein, we developed a novel reverse-phase liquid chromatography coupling quadrupole time-of-flight mass spectrometry (RPLC‒Q-TOF‒MS) method based on the iterative acquisition mode and used the MS/MS mode for deep mining and simultaneous quantification of cholesterol (Chol), CEs and OxCEs in human serum. A mathematical model was used to globally profile 57 molecular species of both CEs and OxCEs in the serum of both healthy volunteers and patients with breast cancer, and the qualitative results were verified based on the retention regularity. An abnormal elevation of OxCEs was found in serum samples of breast cancer patients, where OxCEs were produced by the oxidation of the fatty acyl chain of CE (20:4), such as CE (20:1)+3O, CE (20:2)+2O and CE (20:3)+O, which could be regarded as biomarkers. This comprehensive method for the global profiling of Chol, OxCEs and CEs sheds light on the role OxCEs and CEs play in breast cancer and has enabled the discovery of breast cancer biomarkers.

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:1663

Enthalten in:

Journal of chromatography. A - 1663(2022) vom: 25. Jan., Seite 462764

Sprache:

Englisch

Beteiligte Personen:

Wang, Xincen [VerfasserIn]
Li, He [VerfasserIn]
Zou, Xiao [VerfasserIn]
Yan, Xiong [VerfasserIn]
Cong, Peixu [VerfasserIn]
Li, Hongyan [VerfasserIn]
Wang, Hui [VerfasserIn]
Xue, Changhu [VerfasserIn]
Xu, Jie [VerfasserIn]

Links:

Volltext

Themen:

Biomarkers, Tumor
Breast cancer
Cholesterol Esters
Cholesteryl ester
Iterative acquisition
Journal Article
LC–MS/MS
Oxidized cholesteryl ester
Targeted MS/MS

Anmerkungen:

Date Completed 18.01.2022

Date Revised 18.01.2022

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.chroma.2021.462764

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM334904285