A retrospect of the structure determination of Taka-amylase A
© The Author(s) 2022. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved..
The 3D structure of Taka-amylase A was determined by X-ray crystal analysis at 3 Å resolution by Masao Kakudo's laboratory at the Institute for Protein Research, Osaka University, in 1980. Seven kinds of heavy atom derivatives were used for phase determination. There are three copies of Taka-amylase molecules in the asymmetric unit, which improved the quality of electron density maps, leading to the completion of a molecular model with 478 amino acids. The structure determination process in those days is described briefly.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:171 |
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| Enthalten in: |
Journal of biochemistry - 171(2022), 1 vom: 07. Jan., Seite 17-18 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kusunoki, Masami [VerfasserIn] |
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| Links: |
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| Themen: |
Alpha-Amylases |
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| Anmerkungen: |
Date Completed 10.02.2022 Date Revised 31.05.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1093/jb/mvab137 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a © The Author(s) 2022. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved. | ||
| 520 | |a The 3D structure of Taka-amylase A was determined by X-ray crystal analysis at 3 Å resolution by Masao Kakudo's laboratory at the Institute for Protein Research, Osaka University, in 1980. Seven kinds of heavy atom derivatives were used for phase determination. There are three copies of Taka-amylase molecules in the asymmetric unit, which improved the quality of electron density maps, leading to the completion of a molecular model with 478 amino acids. The structure determination process in those days is described briefly | ||
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