CRISPR/Cas9 editing in conditionally immortalized HoxB8 cells for studying gene regulation in mouse dendritic cells

© 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH..

HoxB8 multipotent progenitors (MPP) are obtained by expression of the estrogen receptor hormone binding domain (ERHBD) HoxB8 fusion gene in mouse BM cells. HoxB8 MPP generate (i) the full complement of DC subsets (cDC1, cDC2, and pDC) in vitro and in vivo and (ii) allow CRISPR/Cas9-mediated gene editing, for example, generating homozygous deletions in cis-acting DNA elements at high precision, and (iii) efficient gene repression by dCas9-KRAB for studying gene regulation in DC differentiation.

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:52

Enthalten in:

European journal of immunology - 52(2022), 11 vom: 01. Nov., Seite 1859-1862

Sprache:

Englisch

Beteiligte Personen:

Xu, Huaming [VerfasserIn]
Look, Thomas [VerfasserIn]
Prithiviraj, Sujeethkumar [VerfasserIn]
Lennartz, Daniel [VerfasserIn]
Cáceres, Manuel Delgado [VerfasserIn]
Götz, Katrin [VerfasserIn]
Wanek, Paul [VerfasserIn]
Häcker, Hans [VerfasserIn]
Kramann, Rafael [VerfasserIn]
Seré, Kristin [VerfasserIn]
Zenke, Martin [VerfasserIn]

Links:

Volltext

Themen:

CRISPR/Cas
DCas9
Dendritic cells
Gene regulation
Homeodomain Proteins
HoxB8
Hoxb8 protein, mouse
Journal Article
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 07.11.2022

Date Revised 27.12.2022

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1002/eji.202149482

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM333634616