CRISPR/Cas9 editing in conditionally immortalized HoxB8 cells for studying gene regulation in mouse dendritic cells
© 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH..
HoxB8 multipotent progenitors (MPP) are obtained by expression of the estrogen receptor hormone binding domain (ERHBD) HoxB8 fusion gene in mouse BM cells. HoxB8 MPP generate (i) the full complement of DC subsets (cDC1, cDC2, and pDC) in vitro and in vivo and (ii) allow CRISPR/Cas9-mediated gene editing, for example, generating homozygous deletions in cis-acting DNA elements at high precision, and (iii) efficient gene repression by dCas9-KRAB for studying gene regulation in DC differentiation.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2022 |
---|---|
Erschienen: |
2022 |
Enthalten in: |
Zur Gesamtaufnahme - volume:52 |
---|---|
Enthalten in: |
European journal of immunology - 52(2022), 11 vom: 01. Nov., Seite 1859-1862 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Xu, Huaming [VerfasserIn] |
---|
Links: |
---|
Themen: |
CRISPR/Cas |
---|
Anmerkungen: |
Date Completed 07.11.2022 Date Revised 27.12.2022 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1002/eji.202149482 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM333634616 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM333634616 | ||
003 | DE-627 | ||
005 | 20231225222043.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2022 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1002/eji.202149482 |2 doi | |
028 | 5 | 2 | |a pubmed24n1112.xml |
035 | |a (DE-627)NLM333634616 | ||
035 | |a (NLM)34826338 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Xu, Huaming |e verfasserin |4 aut | |
245 | 1 | 0 | |a CRISPR/Cas9 editing in conditionally immortalized HoxB8 cells for studying gene regulation in mouse dendritic cells |
264 | 1 | |c 2022 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 07.11.2022 | ||
500 | |a Date Revised 27.12.2022 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2021 The Authors. European Journal of Immunology published by Wiley-VCH GmbH. | ||
520 | |a HoxB8 multipotent progenitors (MPP) are obtained by expression of the estrogen receptor hormone binding domain (ERHBD) HoxB8 fusion gene in mouse BM cells. HoxB8 MPP generate (i) the full complement of DC subsets (cDC1, cDC2, and pDC) in vitro and in vivo and (ii) allow CRISPR/Cas9-mediated gene editing, for example, generating homozygous deletions in cis-acting DNA elements at high precision, and (iii) efficient gene repression by dCas9-KRAB for studying gene regulation in DC differentiation | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a CRISPR/Cas | |
650 | 4 | |a Dendritic cells | |
650 | 4 | |a HoxB8 | |
650 | 4 | |a dCas9 | |
650 | 4 | |a gene regulation | |
650 | 7 | |a Hoxb8 protein, mouse |2 NLM | |
650 | 7 | |a Homeodomain Proteins |2 NLM | |
700 | 1 | |a Look, Thomas |e verfasserin |4 aut | |
700 | 1 | |a Prithiviraj, Sujeethkumar |e verfasserin |4 aut | |
700 | 1 | |a Lennartz, Daniel |e verfasserin |4 aut | |
700 | 1 | |a Cáceres, Manuel Delgado |e verfasserin |4 aut | |
700 | 1 | |a Götz, Katrin |e verfasserin |4 aut | |
700 | 1 | |a Wanek, Paul |e verfasserin |4 aut | |
700 | 1 | |a Häcker, Hans |e verfasserin |4 aut | |
700 | 1 | |a Kramann, Rafael |e verfasserin |4 aut | |
700 | 1 | |a Seré, Kristin |e verfasserin |4 aut | |
700 | 1 | |a Zenke, Martin |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t European journal of immunology |d 1971 |g 52(2022), 11 vom: 01. Nov., Seite 1859-1862 |w (DE-627)NLM000108723 |x 1521-4141 |7 nnns |
773 | 1 | 8 | |g volume:52 |g year:2022 |g number:11 |g day:01 |g month:11 |g pages:1859-1862 |
856 | 4 | 0 | |u http://dx.doi.org/10.1002/eji.202149482 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 52 |j 2022 |e 11 |b 01 |c 11 |h 1859-1862 |