Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain
INTRODUCTION: Trypanosoma cruzi infection triggers an inflammatory process with exacerbated production of cytokines that stimulate inflammatory and anti-inflammatory signals, including the efferent anti-inflammatory signal known as the anti-inflammatory cholinergic pathway. Thus, the use of anticholinesterase drugs, such as galantamine, could minimize the inflammatory process caused by this disease.
METHODS: For the study at 30, 60, and 90 days, 120 Swiss mice were divided into three groups. Each group was subdivided into four subgroups: uninfected/untreated (CTRL), uninfected/treated (GAL), infected/untreated (INF), and infected/treated (GAL/INF). The infected groups were inoculated intraperitoneally with 0.1 ml of mouse blood containing 5 × 104 trypomastigote forms of the T. cruzi QM2 strain. The galantamine-treated groups received 5 mg/kg of galantamine orally, through pipetting. From each subgroup, the parameters of parasitemia, histopathological analysis, butyrylcholinesterase activity (BuChE), and functional study of the colon were evaluated.
RESULTS: BuChE performance was observed when AChE was suppressed, with increased activity in the GAL/INF group similar to the INF group on the 30th day post infection, thus corroborating the absence of a significant difference in parasitic curves and histopathological analysis.
CONCLUSIONS: The presence of an inflammatory process and nests of amastigotes, as well as evidence of reactivity to ACh and NOR, suggest that galantamine did not interfere with the colonic inflammatory response or even in colonic tissue parasitism at this stage of Chagas disease.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:54 |
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| Enthalten in: |
Revista da Sociedade Brasileira de Medicina Tropical - 54(2021) vom: 26., Seite e0201 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Camargo, Lucas Fadel [VerfasserIn] |
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| Links: |
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| Themen: |
0D3Q044KCA |
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| Anmerkungen: |
Date Completed 18.11.2021 Date Revised 04.04.2024 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1590/0037-8682-0201-2021 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM333249402 |
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| 245 | 1 | 0 | |a Influence of galantamine in the inflammatory process and tissular lesions caused by Trypanosoma cruzi QM2 strain |
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| 500 | |a Date Revised 04.04.2024 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a INTRODUCTION: Trypanosoma cruzi infection triggers an inflammatory process with exacerbated production of cytokines that stimulate inflammatory and anti-inflammatory signals, including the efferent anti-inflammatory signal known as the anti-inflammatory cholinergic pathway. Thus, the use of anticholinesterase drugs, such as galantamine, could minimize the inflammatory process caused by this disease | ||
| 520 | |a METHODS: For the study at 30, 60, and 90 days, 120 Swiss mice were divided into three groups. Each group was subdivided into four subgroups: uninfected/untreated (CTRL), uninfected/treated (GAL), infected/untreated (INF), and infected/treated (GAL/INF). The infected groups were inoculated intraperitoneally with 0.1 ml of mouse blood containing 5 × 104 trypomastigote forms of the T. cruzi QM2 strain. The galantamine-treated groups received 5 mg/kg of galantamine orally, through pipetting. From each subgroup, the parameters of parasitemia, histopathological analysis, butyrylcholinesterase activity (BuChE), and functional study of the colon were evaluated | ||
| 520 | |a RESULTS: BuChE performance was observed when AChE was suppressed, with increased activity in the GAL/INF group similar to the INF group on the 30th day post infection, thus corroborating the absence of a significant difference in parasitic curves and histopathological analysis | ||
| 520 | |a CONCLUSIONS: The presence of an inflammatory process and nests of amastigotes, as well as evidence of reactivity to ACh and NOR, suggest that galantamine did not interfere with the colonic inflammatory response or even in colonic tissue parasitism at this stage of Chagas disease | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 7 | |a Butyrylcholinesterase |2 NLM | |
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| 700 | 1 | |a Pinheiro, Guilherme Donzalisky |e verfasserin |4 aut | |
| 700 | 1 | |a Oliveira, Priscilla Bianca de |e verfasserin |4 aut | |
| 700 | 1 | |a Losada, Daniele Moraes |e verfasserin |4 aut | |
| 700 | 1 | |a Chagas, Eduardo Federighi Baisi |e verfasserin |4 aut | |
| 700 | 1 | |a Sperança, Márcia Aparecida |e verfasserin |4 aut | |
| 700 | 1 | |a Chies, Agnaldo Bruno |e verfasserin |4 aut | |
| 700 | 1 | |a Spadella, Maria Angélica |e verfasserin |4 aut | |
| 700 | 1 | |a Martins, Luciamáre Perinetti Alves |e verfasserin |4 aut | |
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