Sequencing 5-Formyluracil in Genomic DNA at Single-Base Resolution
Albeit with low content, 5-formyluracil has been an important modification in genomic DNA. 5-formyluracil was found to be widely distributed among living bodies. Due to the equilibrium of keto-enol form, 5-formyluracil could be base-paired with guanine, thus inducing mutations in DNA. The highly reactive aldehyde group of 5-formyluracil could also cross-link with proteins nearby, preventing gene replication and expression. In certain cancerous tissues, the content of 5-formyluracil was found to be higher than the normal tissues adjacent to the tumor, and 5-formyluracil might be an important potential epigenetic mark. Nevertheless, the lack of a higher resolution sequencing technique has hampered the studies of 5-formyluracil. We adjusted the base-pairing of 5-formyluracil during the PCR amplification by changing the pH. Hence, we adopted the Alkaline Modulated 5-formyluracil Sequencing (AMfU-Seq), a single-base resolution analysis method, to profile 5-formyluracil at the genome scale. We analyzed the distribution of 5-formyluracil in the human thyroid carcinoma cells using AMfU-Seq. This technique can be used in the future investigations of 5-formyluracil.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:93 |
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| Enthalten in: |
Analytical chemistry - 93(2021), 46 vom: 23. Nov., Seite 15445-15451 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yang, Wei [VerfasserIn] |
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| Links: |
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| Themen: |
1195-08-0 |
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| Anmerkungen: |
Date Completed 29.11.2021 Date Revised 29.11.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1021/acs.analchem.1c03339 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM333135121 |
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| 520 | |a Albeit with low content, 5-formyluracil has been an important modification in genomic DNA. 5-formyluracil was found to be widely distributed among living bodies. Due to the equilibrium of keto-enol form, 5-formyluracil could be base-paired with guanine, thus inducing mutations in DNA. The highly reactive aldehyde group of 5-formyluracil could also cross-link with proteins nearby, preventing gene replication and expression. In certain cancerous tissues, the content of 5-formyluracil was found to be higher than the normal tissues adjacent to the tumor, and 5-formyluracil might be an important potential epigenetic mark. Nevertheless, the lack of a higher resolution sequencing technique has hampered the studies of 5-formyluracil. We adjusted the base-pairing of 5-formyluracil during the PCR amplification by changing the pH. Hence, we adopted the Alkaline Modulated 5-formyluracil Sequencing (AMfU-Seq), a single-base resolution analysis method, to profile 5-formyluracil at the genome scale. We analyzed the distribution of 5-formyluracil in the human thyroid carcinoma cells using AMfU-Seq. This technique can be used in the future investigations of 5-formyluracil | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 700 | 1 | |a Han, Shaoqing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xiong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Yafen |e verfasserin |4 aut | |
| 700 | 1 | |a Zou, Guangrong |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Chaoxing |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Muxin |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Xiang |e verfasserin |4 aut | |
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