Antioxidant effect of enamel matrix derivative for early phase of periodontal tissue regeneration in diabetes
© 2021 American Academy of Periodontology..
BACKGROUND: Diabetes involves metabolic disorders in various tissues via hyperglycemia-induced oxidative stress. This study aimed to investigate the antioxidative effect of enamel matrix derivative (EMD) on periodontal regeneration in diabetes.
METHODS: Twenty-two rats were equally divided into streptozotocin (STZ)-induced diabetes or control group. Two months after induction of hyperglycemia, systemic oxidative stress was measured using urinary 8-hydroxy-2'-deoxyguanosine. EMD or saline was applied into the intrabony defects created in the bilateral maxillary molar. mRNA expressions of inflammatory and oxidative stress markers were quantified (n = 6). Histometric analyses and immunohistochemistry of superoxide dismutase-1 (SOD-1) were performed 7 days postoperatively (n = 5). For in vitro experiments, the bone marrow-derived mesenchymal stem cells were isolated from rat femur and cultured in a high glucose (HG) or control medium. Reactive oxygen species (ROS) measurement and alizarin red staining were performed with/without EMD.
RESULTS: Systemic oxidative stress was significantly higher in the diabetic group. The connective tissue attachment and cementum formation were significantly increased at EMD-treated sites in both diabetic and non-diabetic groups. The expression of nicotinamide adenine dinucleotide phosphate oxidase two and four was significantly lower at EMD-treated sites than at EMD-untreated sites in both diabetic and non-diabetic rats. Immunohistochemistry showed significantly higher SOD-1 expression at the EMD-treated site. In vitro, HG culture had significantly higher ROS production compared with control, which was downregulated by EMD. EMD treatment significantly recovered the impaired calcification in HG.
CONCLUSION: EMD promoted early-phase wound healing and periodontal tissue regeneration in the surgically created bony defect of STZ-induced diabetic rat by suppressing hyperglycemia-induced oxidative stress.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:93 |
|---|---|
| Enthalten in: |
Journal of periodontology - 93(2022), 8 vom: 12. Aug., Seite 1206-1217 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Takeda, Kohei [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 18.08.2022 Date Revised 18.09.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/JPER.21-0413 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM333114744 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM333114744 | ||
| 003 | DE-627 | ||
| 005 | 20231225220956.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/JPER.21-0413 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1110.xml |
| 035 | |a (DE-627)NLM333114744 | ||
| 035 | |a (NLM)34773707 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Takeda, Kohei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Antioxidant effect of enamel matrix derivative for early phase of periodontal tissue regeneration in diabetes |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 18.08.2022 | ||
| 500 | |a Date Revised 18.09.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 American Academy of Periodontology. | ||
| 520 | |a BACKGROUND: Diabetes involves metabolic disorders in various tissues via hyperglycemia-induced oxidative stress. This study aimed to investigate the antioxidative effect of enamel matrix derivative (EMD) on periodontal regeneration in diabetes | ||
| 520 | |a METHODS: Twenty-two rats were equally divided into streptozotocin (STZ)-induced diabetes or control group. Two months after induction of hyperglycemia, systemic oxidative stress was measured using urinary 8-hydroxy-2'-deoxyguanosine. EMD or saline was applied into the intrabony defects created in the bilateral maxillary molar. mRNA expressions of inflammatory and oxidative stress markers were quantified (n = 6). Histometric analyses and immunohistochemistry of superoxide dismutase-1 (SOD-1) were performed 7 days postoperatively (n = 5). For in vitro experiments, the bone marrow-derived mesenchymal stem cells were isolated from rat femur and cultured in a high glucose (HG) or control medium. Reactive oxygen species (ROS) measurement and alizarin red staining were performed with/without EMD | ||
| 520 | |a RESULTS: Systemic oxidative stress was significantly higher in the diabetic group. The connective tissue attachment and cementum formation were significantly increased at EMD-treated sites in both diabetic and non-diabetic groups. The expression of nicotinamide adenine dinucleotide phosphate oxidase two and four was significantly lower at EMD-treated sites than at EMD-untreated sites in both diabetic and non-diabetic rats. Immunohistochemistry showed significantly higher SOD-1 expression at the EMD-treated site. In vitro, HG culture had significantly higher ROS production compared with control, which was downregulated by EMD. EMD treatment significantly recovered the impaired calcification in HG | ||
| 520 | |a CONCLUSION: EMD promoted early-phase wound healing and periodontal tissue regeneration in the surgically created bony defect of STZ-induced diabetic rat by suppressing hyperglycemia-induced oxidative stress | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a antioxidant | |
| 650 | 4 | |a diabetes mellitus | |
| 650 | 4 | |a oxidative stress | |
| 650 | 4 | |a periodontal regeneration | |
| 650 | 4 | |a wound healing | |
| 650 | 7 | |a Antioxidants |2 NLM | |
| 650 | 7 | |a Dental Enamel Proteins |2 NLM | |
| 650 | 7 | |a Reactive Oxygen Species |2 NLM | |
| 650 | 7 | |a Superoxide Dismutase |2 NLM | |
| 650 | 7 | |a EC 1.15.1.1 |2 NLM | |
| 700 | 1 | |a Mizutani, Koji |e verfasserin |4 aut | |
| 700 | 1 | |a Matsuura, Takanori |e verfasserin |4 aut | |
| 700 | 1 | |a Kido, Daisuke |e verfasserin |4 aut | |
| 700 | 1 | |a Mikami, Risako |e verfasserin |4 aut | |
| 700 | 1 | |a Buranasin, Prima |e verfasserin |4 aut | |
| 700 | 1 | |a Saito, Natsumi |e verfasserin |4 aut | |
| 700 | 1 | |a Kominato, Hiromi |e verfasserin |4 aut | |
| 700 | 1 | |a Takemura, Shu |e verfasserin |4 aut | |
| 700 | 1 | |a Nakagawa, Keita |e verfasserin |4 aut | |
| 700 | 1 | |a Iwata, Takanori |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of periodontology |d 1970 |g 93(2022), 8 vom: 12. Aug., Seite 1206-1217 |w (DE-627)NLM000139386 |x 1943-3670 |7 nnns |
| 773 | 1 | 8 | |g volume:93 |g year:2022 |g number:8 |g day:12 |g month:08 |g pages:1206-1217 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/JPER.21-0413 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 93 |j 2022 |e 8 |b 12 |c 08 |h 1206-1217 | ||