Characterization of Constitutive Promoters for the Elicitation of Secondary Metabolites in Myxobacteria

Genome mining has revealed that myxobacteria contain a myriad of cryptic biosynthetic gene clusters (BGCs). Here, we report the characterization of a panel of myxobacterial promoters with variable strength that are applicable in the engineering of BGCs in myxobacteria. The screened strongest constitutive promoter was used to efficiently enhance the expression of two complex BGCs governing the biosynthesis of myxochromide and DKxanthene in the model myxobacterium Myxococcus xanthus DK1622. We also showcased the combination of promoter engineering and MS2-based spectral networking as an effective strategy to shed light on the previously overlooked chemistry in the family of myxochromide-type lipopeptides. The enriched promoter library substantially expanded the synthetic biology toolkit available for myxobacteria.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:10

Enthalten in:

ACS synthetic biology - 10(2021), 11 vom: 19. Nov., Seite 2904-2909

Sprache:

Englisch

Beteiligte Personen:

Hu, Wei-Feng [VerfasserIn]
Niu, Luo [VerfasserIn]
Yue, Xin-Jing [VerfasserIn]
Zhu, Le-Le [VerfasserIn]
Hu, Wei [VerfasserIn]
Li, Yue-Zhong [VerfasserIn]
Wu, Changsheng [VerfasserIn]

Links:

Volltext

Themen:

BGCs activation
Bacterial Proteins
Constitutive promoters
Journal Article
Lipopeptides
Metabolomics
Myxobacteria
Myxochromide
Research Support, Non-U.S. Gov't

Anmerkungen:

Date Completed 02.03.2022

Date Revised 02.03.2022

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1021/acssynbio.1c00444

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM332956504