How centrifugation influences the recovery and soy peptone incorporation in hyaluronic acid coils from fermentation

Copyright © 2021 Elsevier B.V. All rights reserved..

Hyaluronic acid from fermentation (bio-HA) is a high-added-value biopolymer with a wide range of applications in aesthetic and medical areas. Bio-HA is a capsular exopolysaccharide highly hydrophilic with a coiled structure and high negative charge density at physiological pH. Centrifugation, currently used to separate microbial cells after fermentation, should significantly affect the bio-HA interactions with the medium components. Although the literature shows various centrifugation conditions for bio-HA, there is no concern about its recovery and characterization. We examined how centrifugation influences its recovery and characterized interactions with soy peptone (SP), used as a nitrogen source. We collected experimental data according to a statistical central composite design (CCD) planning with replicates at the central point with time (8-22 min) and spin (582xg to 12,402xg) as independent variables. The obtained results show bio-HA recoveries ranged from 45 ± 0.5-100% as dosed in the supernatant. The incorporation of amino acids and peptides from SP increased HA coils' density, causing losses due to sedimentation at higher spins and times. Furthermore, SP incorporation also changed the coils' size regarding HA standards of molar mass 106,105 and 104 Da. We conclude centrifugation should significantly impact downstream processing and the total cost of bio-HA production.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:341

Enthalten in:

Journal of biotechnology - 341(2021) vom: 20. Nov., Seite 121-128

Sprache:

Englisch

Beteiligte Personen:

de Souza, Alexandro Barbosa [VerfasserIn]
de Campos Oliveira, Rhelvis [VerfasserIn]
Santana, Maria Helena Andrade [VerfasserIn]

Links:

Volltext

Themen:

9004-61-9
Centrifugation
Fermented broth
Hyaluronic Acid
Hyaluronic acid
Journal Article
Peptones
Quality
Recovery

Anmerkungen:

Date Completed 01.11.2021

Date Revised 01.11.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.jbiotec.2021.09.015

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM331391546