A pH-engineering regenerative DNA tetrahedron ECL biosensor for the assay of SARS-CoV-2 RdRp gene based on CRISPR/Cas12a trans-activity
© 2021 Elsevier B.V. All rights reserved..
In this work, we constructed an exonuclease III cleavage reaction-based isothermal amplification of nucleic acids with CRISPR/Cas12a-mediated pH-induced regenerative Electrochemiluminescence (ECL) biosensor for ultrasensitive and specific detection of SARS-CoV-2 nucleic acids for SARS-CoV-2 diagnosis. The triple-stranded nucleic acid in this biosensor has an extreme dependence on pH, which makes our constructed biosensor reproducible. This is essential for effective large-scale screening of SARS-CoV-2 in areas where resources are currently relatively scarce. Using this pH-induced regenerative biosensor, we detected the SARS-CoV-2 RdRp gene with a detection limit of 43.70 aM. In addition, the detection system has good stability and reproducibility, and we expect that this method may provide a potential platform for the diagnosis of COVID-19.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:429 |
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| Enthalten in: |
Chemical engineering journal (Lausanne, Switzerland : 1996) - 429(2022) vom: 01. Feb., Seite 132472 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Kai [VerfasserIn] |
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| Links: |
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| Themen: |
CRISPR/Cas12a |
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| Anmerkungen: |
Date Revised 21.12.2022 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.cej.2021.132472 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM330811223 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2021 Elsevier B.V. All rights reserved. | ||
| 520 | |a In this work, we constructed an exonuclease III cleavage reaction-based isothermal amplification of nucleic acids with CRISPR/Cas12a-mediated pH-induced regenerative Electrochemiluminescence (ECL) biosensor for ultrasensitive and specific detection of SARS-CoV-2 nucleic acids for SARS-CoV-2 diagnosis. The triple-stranded nucleic acid in this biosensor has an extreme dependence on pH, which makes our constructed biosensor reproducible. This is essential for effective large-scale screening of SARS-CoV-2 in areas where resources are currently relatively scarce. Using this pH-induced regenerative biosensor, we detected the SARS-CoV-2 RdRp gene with a detection limit of 43.70 aM. In addition, the detection system has good stability and reproducibility, and we expect that this method may provide a potential platform for the diagnosis of COVID-19 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a CRISPR/Cas12a | |
| 650 | 4 | |a DNA tetrahedron | |
| 650 | 4 | |a ECL | |
| 650 | 4 | |a Regenerative Biosensor | |
| 650 | 4 | |a SARS-CoV-2 RdRp gene | |
| 650 | 4 | |a Triple-helix | |
| 700 | 1 | |a Fan, Zhenqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Ding, Yuedi |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Minhao |e verfasserin |4 aut | |
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