Bottom-up assembly of biomedical relevant fully synthetic extracellular vesicles
Extracellular vesicles (EVs) are fundamental for intercellular communication and influence nearly every process in cell physiology. However, because of their intricate molecular complexity, quantitative knowledge on their signaling mechanisms is missing, particularly impeding their therapeutic application. We used a complementary and quantitative engineering approach based on sequential synthetic bottom-up assembly of fully functional EVs with precisely controlled lipid, protein, and RNA composition. We show that the functionalities of synthetic EVs are analogous to natural EVs and demonstrate their programmable therapeutic administration for wound healing and neovascularization therapy. We apply transcriptome profiling to systematically decode synergistic effects between individual EV constituents, enabling analytical dissection and a fundamental understanding of EV signaling.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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| Enthalten in: |
Science advances - 7(2021), 36 vom: 03. Sept., Seite eabg6666 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Staufer, Oskar [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 04.04.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1126/sciadv.abg6666 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM330590537 |
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| 520 | |a Extracellular vesicles (EVs) are fundamental for intercellular communication and influence nearly every process in cell physiology. However, because of their intricate molecular complexity, quantitative knowledge on their signaling mechanisms is missing, particularly impeding their therapeutic application. We used a complementary and quantitative engineering approach based on sequential synthetic bottom-up assembly of fully functional EVs with precisely controlled lipid, protein, and RNA composition. We show that the functionalities of synthetic EVs are analogous to natural EVs and demonstrate their programmable therapeutic administration for wound healing and neovascularization therapy. We apply transcriptome profiling to systematically decode synergistic effects between individual EV constituents, enabling analytical dissection and a fundamental understanding of EV signaling | ||
| 650 | 4 | |a Journal Article | |
| 700 | 1 | |a Dietrich, Franziska |e verfasserin |4 aut | |
| 700 | 1 | |a Rimal, Rahul |e verfasserin |4 aut | |
| 700 | 1 | |a Schröter, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Fabritz, Sebastian |e verfasserin |4 aut | |
| 700 | 1 | |a Boehm, Heike |e verfasserin |4 aut | |
| 700 | 1 | |a Singh, Smriti |e verfasserin |4 aut | |
| 700 | 1 | |a Möller, Martin |e verfasserin |4 aut | |
| 700 | 1 | |a Platzman, Ilia |e verfasserin |4 aut | |
| 700 | 1 | |a Spatz, Joachim Pius |e verfasserin |4 aut | |
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