Use of transcriptional slippage for diverse gene expression
We present here an alternative for two-promoter systems ensuring highly diverse expression of several genes from a single promoter. This approach assumes an introduction of a deletion mutation into an A/T homopolymeric run in a gene's proximal part, and employs the transcriptional slippage mechanism for insertion-dependent reinstatement of the proper reading frame by the T7 RNA polymerase.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:68 |
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| Enthalten in: |
Acta biochimica Polonica - 68(2021), 3 vom: 26. Aug., Seite 407-409 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Koscielniak, Dawid [VerfasserIn] |
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| Links: |
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| Themen: |
Bacteriophage T7 RNA polymerase |
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| Anmerkungen: |
Date Completed 27.12.2021 Date Revised 27.12.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.18388/abp.2020_5736 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a We present here an alternative for two-promoter systems ensuring highly diverse expression of several genes from a single promoter. This approach assumes an introduction of a deletion mutation into an A/T homopolymeric run in a gene's proximal part, and employs the transcriptional slippage mechanism for insertion-dependent reinstatement of the proper reading frame by the T7 RNA polymerase | ||
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| 700 | 1 | |a Wons, Ewa |e verfasserin |4 aut | |
| 700 | 1 | |a Sęktas, Marian |e verfasserin |4 aut | |
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