Preparation and Characterization of Quantum Dot-Peptide Conjugates Based on Polyhistidine Tags
© 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature..
Quantum dots (QDs) offer bright and robust photoluminescence among several other advantages in comparison to fluorescent dyes. In order to leverage the advantageous properties of QDs for applications in bioanalysis and imaging, simple and reliable methods for bioconjugation are required. One such method for conjugating peptides to QDs is the use of polyhistidine tags, which spontaneously bind to the surface of QDs. We describe protocols for assembling polyhistidine-tagged peptides to QDs and for characterizing the resultant QD-peptide conjugates. The latter include both electrophoretic and FRET-based protocols for confirming successful peptide assembly, estimating the maximum peptide loading capacity, and measuring the assembly kinetics. Sensors for protease activity and intracellular delivery are briefly noted as prospective applications of QD-peptide conjugates.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2355 |
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| Enthalten in: |
Methods in molecular biology (Clifton, N.J.) - 2355(2021) vom: 12., Seite 175-218 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Krause, Katherine D [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 11.01.2022 Date Revised 11.01.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1007/978-1-0716-1617-8_16 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM329307444 |
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| 520 | |a © 2021. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. | ||
| 520 | |a Quantum dots (QDs) offer bright and robust photoluminescence among several other advantages in comparison to fluorescent dyes. In order to leverage the advantageous properties of QDs for applications in bioanalysis and imaging, simple and reliable methods for bioconjugation are required. One such method for conjugating peptides to QDs is the use of polyhistidine tags, which spontaneously bind to the surface of QDs. We describe protocols for assembling polyhistidine-tagged peptides to QDs and for characterizing the resultant QD-peptide conjugates. The latter include both electrophoretic and FRET-based protocols for confirming successful peptide assembly, estimating the maximum peptide loading capacity, and measuring the assembly kinetics. Sensors for protease activity and intracellular delivery are briefly noted as prospective applications of QD-peptide conjugates | ||
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| 650 | 4 | |a Förster resonance energy transfer (FRET) | |
| 650 | 4 | |a Peptides | |
| 650 | 4 | |a Polyacrylamide gel electrophoresis | |
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| 700 | 1 | |a Rees, Kelly |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Hyungki |e verfasserin |4 aut | |
| 700 | 1 | |a Algar, W Russ |e verfasserin |4 aut | |
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