Biomimetic Biphasic Electrospun Scaffold for Anterior Cruciate Ligament Tissue Engineering

© 2021. The Korean Tissue Engineering and Regenerative Medicine Society..

BACKGROUND: Replacing damaged anterior cruciate ligaments (ACLs) with tissue-engineered artificial ligaments is challenging because ligament scaffolds must have a multiregional structure that can guide stem cell differentiation. Here, we designed a biphasic scaffold and evaluated its effect on human marrow mesenchymal stem cells (MSCs) under dynamic culture conditions as well as rat ACL reconstruction model in vivo.

METHODS: We designed a novel dual-phase electrospinning strategy wherein the scaffolds comprised randomly arranged phases at the two ends and an aligned phase in the middle. The morphological, mechanical properties and scaffold degradation were investigated. MSCs proliferation, adhesion, morphology and fibroblast markers were evaluated under dynamic culturing. This scaffold were tested if they could induce ligament formation using a rodent model in vivo.

RESULTS: Compared with other materials, poly(D,L-lactide-co-glycolide)/poly(ε-caprolactone) (PLGA/PCL) with mass ratio of 1:5 showed appropriate mechanical properties and biodegradability that matched ACLs. After 28 days of dynamic culturing, MSCs were fusiform oriented in the aligned phase and randomly arranged in a paving-stone-like morphology in the random phase. The increased expression of fibroblastic markers demonstrated that only the alignment of nanofibers worked with mechanical stimulation to promote effective fibroblast differentiation. This scaffold was a dense collagenous structure, and there was minimal difference in collagen direction in the orientation phase.

CONCLUSION: Dual-phase electrospun scaffolds had mechanical properties and degradability similar to those of ACLs. They promoted differences in the morphology of MSCs and induced fibroblast differentiation under dynamic culture conditions. Animal experiments showed that ligamentous tissue regenerated well and supported joint stability.

Errataetall:

ErratumIn: Tissue Eng Regen Med. 2021 Sep 4;:. - PMID 34480733

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:18

Enthalten in:

Tissue engineering and regenerative medicine - 18(2021), 5 vom: 06. Okt., Seite 819-830

Sprache:

Englisch

Beteiligte Personen:

Tang, Ya [VerfasserIn]
Tian, Jialiang [VerfasserIn]
Li, Long [VerfasserIn]
Huang, Lin [VerfasserIn]
Shen, Quan [VerfasserIn]
Guo, Shanzhu [VerfasserIn]
Jiang, Yue [VerfasserIn]

Links:

Volltext

Themen:

Anterior cruciate ligament
Biphasic
Electrospun
Journal Article
Research Support, Non-U.S. Gov't
Tissue engineering

Anmerkungen:

Date Completed 13.10.2021

Date Revised 06.08.2022

published: Print-Electronic

ErratumIn: Tissue Eng Regen Med. 2021 Sep 4;:. - PMID 34480733

Citation Status MEDLINE

doi:

10.1007/s13770-021-00376-7

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM328996416