Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida
The expression of 5-HT (serotonin) receptors (sr) was analyzed in the spinal cord and ganglia of 15 human conceptuses (5-10-weeks), and in the 9-week fetus with spina bifida. We used immunohistochemical method to detect sr-positive, apoptotic (caspase-3) and proliferating (Ki-67) cells, double immunofluorescence for co-localization with protein gene peptide (pgp) 9.5 and GFAP, as well as semiquantification and statistical measurements. Following the neurulation process, moderate (sr1 and sr2) and mild (sr3) expression characterized neuroblasts in the spinal cord and ganglia. During further development, sr1 expression gradually increased in the motoneurons, autonomic and sensory neurons, while sr2 and sr3 increased strongly in floor and roof plates. In the ganglia, sr3 expression increased during limited developmental period, while sr1 and sr2 increased throughout the investigated period. Co-expression of sr/pgp 9.5 characterized developing neurons, while sr/GFAP co-localized in the roof plate. In the spinal cord and ganglia of malformed fetus, weaker sr1 and sr2 and stronger sr3 expression accompanied morphological abnormalities. Anomalous roof plate morphology showed an excess of apoptotic and proliferating cells and increased sr3 expression. Our results indicate a human-species specific sr expression pattern, and the importance of sr1 in neuronal differentiation, and sr2 and sr3 in the control of the roof plate morphogenesis in normal and disturbed development.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
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| Enthalten in: |
International journal of molecular sciences - 22(2021), 14 vom: 07. Juli |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Punda, Hrvoje [VerfasserIn] |
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| Links: |
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| Themen: |
333DO1RDJY |
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| Anmerkungen: |
Date Completed 05.08.2021 Date Revised 05.08.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/ijms22147320 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM328438049 |
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| 100 | 1 | |a Punda, Hrvoje |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Expression Pattern of 5-HT (Serotonin) Receptors during Normal Development of the Human Spinal Cord and Ganglia and in Fetus with Cervical Spina Bifida |
| 264 | 1 | |c 2021 | |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The expression of 5-HT (serotonin) receptors (sr) was analyzed in the spinal cord and ganglia of 15 human conceptuses (5-10-weeks), and in the 9-week fetus with spina bifida. We used immunohistochemical method to detect sr-positive, apoptotic (caspase-3) and proliferating (Ki-67) cells, double immunofluorescence for co-localization with protein gene peptide (pgp) 9.5 and GFAP, as well as semiquantification and statistical measurements. Following the neurulation process, moderate (sr1 and sr2) and mild (sr3) expression characterized neuroblasts in the spinal cord and ganglia. During further development, sr1 expression gradually increased in the motoneurons, autonomic and sensory neurons, while sr2 and sr3 increased strongly in floor and roof plates. In the ganglia, sr3 expression increased during limited developmental period, while sr1 and sr2 increased throughout the investigated period. Co-expression of sr/pgp 9.5 characterized developing neurons, while sr/GFAP co-localized in the roof plate. In the spinal cord and ganglia of malformed fetus, weaker sr1 and sr2 and stronger sr3 expression accompanied morphological abnormalities. Anomalous roof plate morphology showed an excess of apoptotic and proliferating cells and increased sr3 expression. Our results indicate a human-species specific sr expression pattern, and the importance of sr1 in neuronal differentiation, and sr2 and sr3 in the control of the roof plate morphogenesis in normal and disturbed development | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a cervical spina bifida | |
| 650 | 4 | |a ganglia | |
| 650 | 4 | |a human development | |
| 650 | 4 | |a serotonin | |
| 650 | 4 | |a spinal cord | |
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| 700 | 1 | |a Filipovic, Natalija |e verfasserin |4 aut | |
| 700 | 1 | |a Kosovic, Ivona |e verfasserin |4 aut | |
| 700 | 1 | |a Benzon, Benjamin |e verfasserin |4 aut | |
| 700 | 1 | |a Ogorevc, Marin |e verfasserin |4 aut | |
| 700 | 1 | |a Bocina, Ivana |e verfasserin |4 aut | |
| 700 | 1 | |a Kolic, Kresimir |e verfasserin |4 aut | |
| 700 | 1 | |a Vukojevic, Katarina |e verfasserin |4 aut | |
| 700 | 1 | |a Saraga-Babic, Mirna |e verfasserin |4 aut | |
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