A Novel Electrochemical Aptasensor for the Ultrasensitive Detection of Adenosine Triphosphate Based on DNA-Templated Copolymers

Adenosine triphosphate (ATP) is a small but significant biological molecule that plays an important role in regulating cellular metabolism processes. Accurate and sensitive analytical techniques for detecting ATP are urgently needed. Herein, a new electrochemical aptasensor was designed in light of DNA-templated copolymers that parallel to the electrode for sensitive gauging of ATP. The ATP aptamer decorated by the electronic medium ferrocene can be regarded as a ″bridge″ connecting two DNA-templated copolymers. When ATP exists, because of the extraordinary binding selectivity of DNA-templated copolymers for target ATP, the rapid electron transfer of ferrocene was beneficial to the sensitive detection of target ATP. Specifically, our parallel DNA copolymers are more stable than upright DNA copolymers and have a faster signal transmission because of the close distance to the electrode; meanwhile, the nonspecific pollution is less. Consequently, the developed novel aptasensor exhibited a wide range of linear response toward ATP that was from 0.1 to 10 000 pM and high selectivity with a detection limit down to 11.5 fM. The electrochemical aptasensor has a broad application prospect in the detection of small biological molecules.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:13

Enthalten in:

ACS applied materials & interfaces - 13(2021), 30 vom: 04. Aug., Seite 35561-35567

Sprache:

Englisch

Beteiligte Personen:

He, Xiaoyan [VerfasserIn]
Dong, Jiandi [VerfasserIn]
Han, Huimin [VerfasserIn]
Sun, Nan [VerfasserIn]
Shi, Wenyu [VerfasserIn]
Lu, Xiong [VerfasserIn]
Jia, Hui [VerfasserIn]
Lu, Xiaoquan [VerfasserIn]

Links:

Volltext

Themen:

8L70Q75FXE
9007-49-2
Adenosine Triphosphate
Adenosine triphosphate
Aptamer
Aptamers, Nucleotide
Benzoates
DNA
DNA-templated copolymers
Ferrocene
Ferrocene dicarboxylic acid
Ferrous Compounds
Hybridization chain reaction
Journal Article
Metallocenes

Anmerkungen:

Date Completed 23.11.2021

Date Revised 23.11.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1021/acsami.1c10173

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM328415170