A Novel Electrochemical Aptasensor for the Ultrasensitive Detection of Adenosine Triphosphate Based on DNA-Templated Copolymers
Adenosine triphosphate (ATP) is a small but significant biological molecule that plays an important role in regulating cellular metabolism processes. Accurate and sensitive analytical techniques for detecting ATP are urgently needed. Herein, a new electrochemical aptasensor was designed in light of DNA-templated copolymers that parallel to the electrode for sensitive gauging of ATP. The ATP aptamer decorated by the electronic medium ferrocene can be regarded as a ″bridge″ connecting two DNA-templated copolymers. When ATP exists, because of the extraordinary binding selectivity of DNA-templated copolymers for target ATP, the rapid electron transfer of ferrocene was beneficial to the sensitive detection of target ATP. Specifically, our parallel DNA copolymers are more stable than upright DNA copolymers and have a faster signal transmission because of the close distance to the electrode; meanwhile, the nonspecific pollution is less. Consequently, the developed novel aptasensor exhibited a wide range of linear response toward ATP that was from 0.1 to 10 000 pM and high selectivity with a detection limit down to 11.5 fM. The electrochemical aptasensor has a broad application prospect in the detection of small biological molecules.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
---|---|
Enthalten in: |
ACS applied materials & interfaces - 13(2021), 30 vom: 04. Aug., Seite 35561-35567 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
He, Xiaoyan [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 23.11.2021 Date Revised 23.11.2021 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1021/acsami.1c10173 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM328415170 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM328415170 | ||
003 | DE-627 | ||
005 | 20231225202903.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1021/acsami.1c10173 |2 doi | |
028 | 5 | 2 | |a pubmed24n1094.xml |
035 | |a (DE-627)NLM328415170 | ||
035 | |a (NLM)34296595 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a He, Xiaoyan |e verfasserin |4 aut | |
245 | 1 | 2 | |a A Novel Electrochemical Aptasensor for the Ultrasensitive Detection of Adenosine Triphosphate Based on DNA-Templated Copolymers |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 23.11.2021 | ||
500 | |a Date Revised 23.11.2021 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Adenosine triphosphate (ATP) is a small but significant biological molecule that plays an important role in regulating cellular metabolism processes. Accurate and sensitive analytical techniques for detecting ATP are urgently needed. Herein, a new electrochemical aptasensor was designed in light of DNA-templated copolymers that parallel to the electrode for sensitive gauging of ATP. The ATP aptamer decorated by the electronic medium ferrocene can be regarded as a ″bridge″ connecting two DNA-templated copolymers. When ATP exists, because of the extraordinary binding selectivity of DNA-templated copolymers for target ATP, the rapid electron transfer of ferrocene was beneficial to the sensitive detection of target ATP. Specifically, our parallel DNA copolymers are more stable than upright DNA copolymers and have a faster signal transmission because of the close distance to the electrode; meanwhile, the nonspecific pollution is less. Consequently, the developed novel aptasensor exhibited a wide range of linear response toward ATP that was from 0.1 to 10 000 pM and high selectivity with a detection limit down to 11.5 fM. The electrochemical aptasensor has a broad application prospect in the detection of small biological molecules | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a DNA-templated copolymers | |
650 | 4 | |a adenosine triphosphate | |
650 | 4 | |a aptamer | |
650 | 4 | |a ferrocene | |
650 | 4 | |a hybridization chain reaction | |
650 | 7 | |a Aptamers, Nucleotide |2 NLM | |
650 | 7 | |a Benzoates |2 NLM | |
650 | 7 | |a Ferrous Compounds |2 NLM | |
650 | 7 | |a Metallocenes |2 NLM | |
650 | 7 | |a ferrocene dicarboxylic acid |2 NLM | |
650 | 7 | |a Adenosine Triphosphate |2 NLM | |
650 | 7 | |a 8L70Q75FXE |2 NLM | |
650 | 7 | |a DNA |2 NLM | |
650 | 7 | |a 9007-49-2 |2 NLM | |
700 | 1 | |a Dong, Jiandi |e verfasserin |4 aut | |
700 | 1 | |a Han, Huimin |e verfasserin |4 aut | |
700 | 1 | |a Sun, Nan |e verfasserin |4 aut | |
700 | 1 | |a Shi, Wenyu |e verfasserin |4 aut | |
700 | 1 | |a Lu, Xiong |e verfasserin |4 aut | |
700 | 1 | |a Jia, Hui |e verfasserin |4 aut | |
700 | 1 | |a Lu, Xiaoquan |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t ACS applied materials & interfaces |d 2009 |g 13(2021), 30 vom: 04. Aug., Seite 35561-35567 |w (DE-627)NLM194100049 |x 1944-8252 |7 nnns |
773 | 1 | 8 | |g volume:13 |g year:2021 |g number:30 |g day:04 |g month:08 |g pages:35561-35567 |
856 | 4 | 0 | |u http://dx.doi.org/10.1021/acsami.1c10173 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 13 |j 2021 |e 30 |b 04 |c 08 |h 35561-35567 |