Heterologous Expression and Purification of CRISPR-Cas12a/Cpf1
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC..
This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification and briefly describes how activity assays can be performed. These protocols can also be used for purification of other Cas12a homologs and the purified proteins can be used for subsequent genome editing experiments. Figure 1. Timeline of activities for the heterologous expression and purification of Francisella novicida Cas12a (FnCas12a) from Escherichia coli.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2018 |
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Erschienen: |
2018 |
Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
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Enthalten in: |
Bio-protocol - 8(2018), 9 vom: 05. Mai, Seite e2842 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Mohanraju, Prarthana [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Revised 10.02.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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doi: |
10.21769/BioProtoc.2842 |
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funding: |
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PPN (Katalog-ID): |
NLM328311537 |
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520 | |a This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification and briefly describes how activity assays can be performed. These protocols can also be used for purification of other Cas12a homologs and the purified proteins can be used for subsequent genome editing experiments. Figure 1. Timeline of activities for the heterologous expression and purification of Francisella novicida Cas12a (FnCas12a) from Escherichia coli | ||
650 | 4 | |a Journal Article | |
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