Heterologous Expression and Purification of CRISPR-Cas12a/Cpf1

Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC..

This protocol provides step by step instructions (Figure 1) for heterologous expression of Francisella novicida Cas12a (previously known as Cpf1) in Escherichia coli. It additionally includes a protocol for high-purity purification and briefly describes how activity assays can be performed. These protocols can also be used for purification of other Cas12a homologs and the purified proteins can be used for subsequent genome editing experiments. Figure 1. Timeline of activities for the heterologous expression and purification of Francisella novicida Cas12a (FnCas12a) from Escherichia coli.

Medienart:

E-Artikel

Erscheinungsjahr:

2018

Erschienen:

2018

Enthalten in:

Zur Gesamtaufnahme - volume:8

Enthalten in:

Bio-protocol - 8(2018), 9 vom: 05. Mai, Seite e2842

Sprache:

Englisch

Beteiligte Personen:

Mohanraju, Prarthana [VerfasserIn]
Van Der Oost, John [VerfasserIn]
Jinek, Martin [VerfasserIn]
Swarts, Daan C [VerfasserIn]

Links:

Volltext

Themen:

CRISPR-Cas
Cas12a
Cpf1
Journal Article
Protein purification

Anmerkungen:

Date Revised 10.02.2022

published: Electronic-eCollection

Citation Status PubMed-not-MEDLINE

doi:

10.21769/BioProtoc.2842

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM328311537