OsPDCD5 negatively regulates plant architecture and grain yield in rice
Plant architecture is an important agronomic trait that affects crop yield. Here, we report that a gene involved in programmed cell death, OsPDCD5, negatively regulates plant architecture and grain yield in rice. We used the CRISPR/Cas9 system to introduce loss-of-function mutations into OsPDCD5 in 11 rice cultivars. Targeted mutagenesis of OsPDCD5 enhanced grain yield and improved plant architecture by increasing plant height and optimizing panicle type and grain shape. Transcriptome analysis showed that OsPDCD5 knockout affected auxin biosynthesis, as well as the gibberellin and cytokinin biosynthesis and signaling pathways. OsPDCD5 interacted directly with OsAGAP, and OsAGAP positively regulated plant architecture and grain yield in rice. Collectively, these findings demonstrate that OsPDCD5 is a promising candidate gene for breeding super rice cultivars with increased yield potential and superior quality.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:118 |
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| Enthalten in: |
Proceedings of the National Academy of Sciences of the United States of America - 118(2021), 29 vom: 20. Juli |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dong, Shiqing [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 08.12.2021 Date Revised 16.01.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1073/pnas.2018799118 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM328124192 |
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| 245 | 1 | 0 | |a OsPDCD5 negatively regulates plant architecture and grain yield in rice |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Plant architecture is an important agronomic trait that affects crop yield. Here, we report that a gene involved in programmed cell death, OsPDCD5, negatively regulates plant architecture and grain yield in rice. We used the CRISPR/Cas9 system to introduce loss-of-function mutations into OsPDCD5 in 11 rice cultivars. Targeted mutagenesis of OsPDCD5 enhanced grain yield and improved plant architecture by increasing plant height and optimizing panicle type and grain shape. Transcriptome analysis showed that OsPDCD5 knockout affected auxin biosynthesis, as well as the gibberellin and cytokinin biosynthesis and signaling pathways. OsPDCD5 interacted directly with OsAGAP, and OsAGAP positively regulated plant architecture and grain yield in rice. Collectively, these findings demonstrate that OsPDCD5 is a promising candidate gene for breeding super rice cultivars with increased yield potential and superior quality | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a OsPDCD5 | |
| 650 | 4 | |a grain yield | |
| 650 | 4 | |a plant architecture | |
| 650 | 4 | |a polar auxin transport | |
| 650 | 4 | |a programmed cell death | |
| 650 | 7 | |a Apoptosis Regulatory Proteins |2 NLM | |
| 650 | 7 | |a Cytokinins |2 NLM | |
| 650 | 7 | |a Gibberellins |2 NLM | |
| 650 | 7 | |a Indoleacetic Acids |2 NLM | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 700 | 1 | |a Dong, Xianxin |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Xiaokang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Fan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Xin, Xiaoyun |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Yuanyi |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Dingyang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jianping |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Zhou |e verfasserin |4 aut | |
| 700 | 1 | |a Niu, Fuan |e verfasserin |4 aut | |
| 700 | 1 | |a Hu, Zejun |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Peiwen |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Liming |e verfasserin |4 aut | |
| 700 | 1 | |a He, Haohua |e verfasserin |4 aut | |
| 700 | 1 | |a Fu, Junru |e verfasserin |4 aut | |
| 700 | 1 | |a Xin, Yeyun |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Yanning |e verfasserin |4 aut | |
| 700 | 1 | |a Mao, Bigang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Bingran |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Jinshui |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Longping |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Xiaojin |e verfasserin |4 aut | |
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