Quantifying Staphylococcus aureus Membrane Potential Using Flow Cytometry

© 2021. Springer Science+Business Media, LLC, part of Springer Nature..

Quantifying fluorescent markers in cell populations using flow cytometry has been a powerful technological advance. Fluorescent properties of cyanine dyes coupled with flow cytometry allow investigators to monitor the membrane potential (MP), an important component of the proton motive force (PMF). MP (or ΔΨ) is the electrical potential across the cell membrane. The other component of the PMF is ΔpH, or the difference in interior and exterior proton concentrations. MP plays a critical role in bacterial physiology. In Staphylococcus aureus, MP is required for generation of ATP, regulating autolytic activity, maintaining ion homeostasis, and resistance to some classes of antibiotics. This protocol exploits unique spectral and physical properties of the cyanine-based molecule diethyloxacarbocyanine iodide, or DiOC, and flow cytometry technology to quantify MP in S. aureus. This assay has been used by researchers to define the electron transport chain of S. aureus as well as determine how intrinsic and extrinsic factors affect MP.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:2341

Enthalten in:

Methods in molecular biology (Clifton, N.J.) - 2341(2021) vom: 15., Seite 95-101

Sprache:

Englisch

Beteiligte Personen:

Hammer, Neal D [VerfasserIn]

Links:

Volltext

Themen:

Carbocyanines
Coloring Agents
DiOC
Flow cytometry
Journal Article
Membrane potential
Proton motive force (PMF)
Respiration
Staphylococcus aureus

Anmerkungen:

Date Completed 25.08.2021

Date Revised 25.08.2021

published: Print

Citation Status MEDLINE

doi:

10.1007/978-1-0716-1550-8_12

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM328099686