The gut brain in a dish : Murine primary enteric nervous system cell cultures
© 2021 The Authors. Neurogastroenterology & Motility published by John Wiley & Sons Ltd..
BACKGROUND: The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease.
PURPOSE: This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
|---|---|
| Enthalten in: |
Neurogastroenterology and motility - 34(2022), 2 vom: 30. Feb., Seite e14215 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Schonkeren, Simone L [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Adult mouse |
|---|
| Anmerkungen: |
Date Completed 04.04.2022 Date Revised 31.07.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/nmo.14215 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM327821086 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM327821086 | ||
| 003 | DE-627 | ||
| 005 | 20231225201630.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/nmo.14215 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1092.xml |
| 035 | |a (DE-627)NLM327821086 | ||
| 035 | |a (NLM)34236124 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Schonkeren, Simone L |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The gut brain in a dish |b Murine primary enteric nervous system cell cultures |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 04.04.2022 | ||
| 500 | |a Date Revised 31.07.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 The Authors. Neurogastroenterology & Motility published by John Wiley & Sons Ltd. | ||
| 520 | |a BACKGROUND: The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease | ||
| 520 | |a PURPOSE: This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Review | |
| 650 | 4 | |a adult mouse | |
| 650 | 4 | |a enteric glial cells | |
| 650 | 4 | |a enteric neurons | |
| 650 | 4 | |a primary ENS culture | |
| 650 | 4 | |a protocol | |
| 700 | 1 | |a Küthe, Tara T |e verfasserin |4 aut | |
| 700 | 1 | |a Idris, Musa |e verfasserin |4 aut | |
| 700 | 1 | |a Bon-Frauches, Ana C |e verfasserin |4 aut | |
| 700 | 1 | |a Boesmans, Werend |e verfasserin |4 aut | |
| 700 | 1 | |a Melotte, Veerle |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Neurogastroenterology and motility |d 1994 |g 34(2022), 2 vom: 30. Feb., Seite e14215 |w (DE-627)NLM075187337 |x 1365-2982 |7 nnns |
| 773 | 1 | 8 | |g volume:34 |g year:2022 |g number:2 |g day:30 |g month:02 |g pages:e14215 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/nmo.14215 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 34 |j 2022 |e 2 |b 30 |c 02 |h e14215 | ||