Characterization of a biofilm formed by Fusarium oxysporum on the human nails

© 2021 the International Society of Dermatology..

BACKGROUND: Fusarium spp. has been considered as an onychomycosis agent, but little is known about the etiopathogenesis of fusarial onychomycosis; thus, the objective of this study was to characterize the fungal-nail interaction and the consequences of the nail infection process by Fusarium oxysporum using the human nail, in an ex vivo model.

METHODS: The kinetic of biofilm production and infection by F. oxysporum using the nail as the only nutritional source were evaluated by scanning electron microscopy, number of culturable cells, metabolic activity, characterization of extracellular matrix, spectroscopy and histopathology analyses.

RESULTS: After evaluating the biofilm kinetic over 7 days using different parameters and techniques, it was possible to characterize the Fusarium-nail interaction.

CONCLUSIONS: This study is a part of a big project aiming to clarify the fusarial pathogenesis and contributes to proving F. oxysporum is able to adapt, grow, develop, and form a biofilm on healthy human nails, which are crucial steps for the invasion process.

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:61

Enthalten in:

International journal of dermatology - 61(2022), 2 vom: 08. Feb., Seite 191-198

Sprache:

Englisch

Beteiligte Personen:

Veiga, Flavia Franco [VerfasserIn]
de Castro-Hoshino, Lidiane Vizioli [VerfasserIn]
Sato, Francielle [VerfasserIn]
Baesso, Mauro Luciano [VerfasserIn]
Silva, Sónia [VerfasserIn]
Negri, Melyssa [VerfasserIn]
Svidzinski, Terezinha Inez Estivalet [VerfasserIn]

Links:

Volltext

Themen:

Journal Article

Anmerkungen:

Date Completed 12.01.2022

Date Revised 12.01.2022

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1111/ijd.15747

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM327817216