A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development
Biomechanical forces intimately contribute to cardiac morphogenesis. However, volumetric imaging to investigate the cardiac mechanics with high temporal and spatial resolution remains an imaging challenge. We hereby integrated light-field microscopy (LFM) with light-sheet fluorescence microscopy (LSFM), coupled with a retrospective gating method, to simultaneously access myocardial contraction and intracardiac blood flow at 200 volumes per second. While LSFM allows for the reconstruction of the myocardial function, LFM enables instantaneous acquisition of the intracardiac blood cells traversing across the valves. We further adopted deformable image registration to quantify the ventricular wall displacement and particle tracking velocimetry to monitor intracardiac blood flow. The integration of LFM and LSFM enabled the time-dependent tracking of the individual blood cells and the differential rates of segmental wall displacement during a cardiac cycle. Taken together, we demonstrated a hybrid system, coupled with our image analysis pipeline, to simultaneously capture the myocardial wall motion with intracardiac blood flow during cardiac development.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:17 |
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| Enthalten in: |
PLoS computational biology - 17(2021), 7 vom: 06. Juli, Seite e1009175 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Zhaoqiang [VerfasserIn] |
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| Anmerkungen: |
Date Completed 25.10.2021 Date Revised 21.04.2022 published: Electronic-eCollection figshare: 10.6084/m9.figshare.14813628 Citation Status MEDLINE |
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| doi: |
10.1371/journal.pcbi.1009175 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM327750227 |
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| 100 | 1 | |a Wang, Zhaoqiang |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A hybrid of light-field and light-sheet imaging to study myocardial function and intracardiac blood flow during zebrafish development |
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| 500 | |a Date Completed 25.10.2021 | ||
| 500 | |a Date Revised 21.04.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a figshare: 10.6084/m9.figshare.14813628 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Biomechanical forces intimately contribute to cardiac morphogenesis. However, volumetric imaging to investigate the cardiac mechanics with high temporal and spatial resolution remains an imaging challenge. We hereby integrated light-field microscopy (LFM) with light-sheet fluorescence microscopy (LSFM), coupled with a retrospective gating method, to simultaneously access myocardial contraction and intracardiac blood flow at 200 volumes per second. While LSFM allows for the reconstruction of the myocardial function, LFM enables instantaneous acquisition of the intracardiac blood cells traversing across the valves. We further adopted deformable image registration to quantify the ventricular wall displacement and particle tracking velocimetry to monitor intracardiac blood flow. The integration of LFM and LSFM enabled the time-dependent tracking of the individual blood cells and the differential rates of segmental wall displacement during a cardiac cycle. Taken together, we demonstrated a hybrid system, coupled with our image analysis pipeline, to simultaneously capture the myocardial wall motion with intracardiac blood flow during cardiac development | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Satta, Sandro |e verfasserin |4 aut | |
| 700 | 1 | |a Roustaei, Mehrdad |e verfasserin |4 aut | |
| 700 | 1 | |a Fei, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Hsiai, Tzung K |e verfasserin |4 aut | |
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