Mechanical stimulation of chondrocytes regulates HIF-1α under hypoxic conditions
Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved..
We investigated the effects of hypoxia-inducible factor (HIF)-1α on articular cartilage under mechanical stimulation and the associated mechanisms. Chondrocytes, isolated from articular cartilage from the knee, hip, and shoulder joints of Wistar rats, were subjected to 20 % tensile stress under hypoxic (5% O2) conditions for 24 h. HIF-1α and aggrecan expression was significantly enhanced with mechanical stimulation under hypoxia but not significantly altered with mechanical stimulation under normoxia. The nuclear translocation of HIF-1α was enhanced by mechanical stress under hypoxia. Under both normoxia and hypoxia, a disintegrin and metalloproteinase with thrombospondin motifs (ADAM-TS) 5 expression was significantly reduced with mechanical stimulation compared to that in the group without mechanical stimulation. However, HIF-1α knockdown mitigated changes in aggrecan and ADAM-TS5 expression mediated by mechanical stimulation under hypoxia. The effects of treadmill running on HIF-1α production in the articular cartilage of rat knee joints were also analyzed. HIF-1α production increased in the moderate running group and decreased to the same levels as those in the control group in the excessive running group. This suggests that HIF-1α regulates aggrecan and ADAM-TS5 expression in response to mechanical stimulation under hypoxia and general mechanical stimulation in articular cartilage under hypoxia, while controlling cartilage homeostasis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:71 |
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| Enthalten in: |
Tissue & cell - 71(2021) vom: 01. Aug., Seite 101574 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shimomura, Seiji [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.01.2022 Date Revised 07.01.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.tice.2021.101574 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a We investigated the effects of hypoxia-inducible factor (HIF)-1α on articular cartilage under mechanical stimulation and the associated mechanisms. Chondrocytes, isolated from articular cartilage from the knee, hip, and shoulder joints of Wistar rats, were subjected to 20 % tensile stress under hypoxic (5% O2) conditions for 24 h. HIF-1α and aggrecan expression was significantly enhanced with mechanical stimulation under hypoxia but not significantly altered with mechanical stimulation under normoxia. The nuclear translocation of HIF-1α was enhanced by mechanical stress under hypoxia. Under both normoxia and hypoxia, a disintegrin and metalloproteinase with thrombospondin motifs (ADAM-TS) 5 expression was significantly reduced with mechanical stimulation compared to that in the group without mechanical stimulation. However, HIF-1α knockdown mitigated changes in aggrecan and ADAM-TS5 expression mediated by mechanical stimulation under hypoxia. The effects of treadmill running on HIF-1α production in the articular cartilage of rat knee joints were also analyzed. HIF-1α production increased in the moderate running group and decreased to the same levels as those in the control group in the excessive running group. This suggests that HIF-1α regulates aggrecan and ADAM-TS5 expression in response to mechanical stimulation under hypoxia and general mechanical stimulation in articular cartilage under hypoxia, while controlling cartilage homeostasis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Arthritis | |
| 650 | 4 | |a Chondrocyte | |
| 650 | 4 | |a Hypoxia | |
| 650 | 4 | |a Mechanical stimulation | |
| 650 | 4 | |a Osteoarthritis | |
| 650 | 4 | |a Treadmill | |
| 650 | 7 | |a Hif1a protein, rat |2 NLM | |
| 650 | 7 | |a Hypoxia-Inducible Factor 1, alpha Subunit |2 NLM | |
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| 700 | 1 | |a Inoue, Hiroaki |e verfasserin |4 aut | |
| 700 | 1 | |a Arai, Yuji |e verfasserin |4 aut | |
| 700 | 1 | |a Nakagawa, Shuji |e verfasserin |4 aut | |
| 700 | 1 | |a Fujii, Yuta |e verfasserin |4 aut | |
| 700 | 1 | |a Kishida, Tsunao |e verfasserin |4 aut | |
| 700 | 1 | |a Shin-Ya, Masaharu |e verfasserin |4 aut | |
| 700 | 1 | |a Ichimaru, Shohei |e verfasserin |4 aut | |
| 700 | 1 | |a Tsuchida, Shinji |e verfasserin |4 aut | |
| 700 | 1 | |a Mazda, Osam |e verfasserin |4 aut | |
| 700 | 1 | |a Takahashi, Kenji |e verfasserin |4 aut | |
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