Overexpression of OsPHR3 improves growth traits and facilitates nitrogen use efficiency under low phosphate condition

Copyright © 2021. Published by Elsevier Masson SAS..

Phosphorus (P) and nitrogen (N) are both essential macronutrients for maintaining plant growth and development. In rice (Oryza sativa L.), OsPHR3 is one of the four paralogs of PHR1, which acts as a central regulator of phosphate (Pi) homeostasis, as well being involved in N homeostasis. However, the functions of OsPHR3 in N utilization under different Pi conditions have yet to be fully studied. In this study, we aimed to dissect the effect of OsPHR3-overexpression on N utilization under Pi deficient regimes. Biochemical, molecular and physiological assays were performed to determine the N-influx, translocation, and accumulation in OsPHR3-overexpressing rice lines, grown under Pi-sufficient and -deficient conditions, in both hydroponic and soil systems. Furthermore, important agronomic traits of these plants were also evaluated. The overexpression of OsPHR3 increased N uptake under Pi stress regimes. Increased N uptake also elevated total N concentrations in these plants by inducing N transporter genes expression. Furthermore, overexpression of OsPHR3 increased N use efficiency, 1000-grain weight and grain yield under different Pi conditions. We established new findings that OsPHR3-overexpression facilitates N utilization under Pi deficient conditions. This will help achieving higher yields by coordinating the utilization of N and P.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:166

Enthalten in:

Plant physiology and biochemistry : PPB - 166(2021) vom: 01. Sept., Seite 712-722

Sprache:

Englisch

Beteiligte Personen:

Sun, Yafei [VerfasserIn]
Hu, Zhi [VerfasserIn]
Wang, Xiaowen [VerfasserIn]
Shen, Xing [VerfasserIn]
Hu, Siwen [VerfasserIn]
Yan, Yan [VerfasserIn]
Kant, Surya [VerfasserIn]
Xu, Guohua [VerfasserIn]
Xue, Yong [VerfasserIn]
Sun, Shubin [VerfasserIn]

Links:

Volltext

Themen:

Journal Article
N762921K75
Nitrogen
Nitrogen use efficiency
OsPHR3
Phosphate
Phosphates
Pi deficiency
Plant Proteins
Transcription Factors
Transgenic

Anmerkungen:

Date Completed 07.09.2021

Date Revised 07.09.2021

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.plaphy.2021.06.041

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM327614552