A counting-strategy together with a spatial structured model describes RNA polymerase and ribosome availability in Escherichia coli
Copyright © 2021 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved..
The allocation of resources during bacterial growth is strongly related to the availability of ribosomes and RNA polymerase molecules. Here, coarse-grained models offer a promising start due to their simple structure and the limited number of kinetic parameters. Based on published data sets for proteome and mRNA data in Escherichia coli, and together with mass balance equations describing gene expression, we are able to calculate the number of active molecules (that is, the number of ribosomes that are currently translating nascent and mature mRNA, as well as the number of RNA polymerase molecules on the DNA). This information is a prerequisite for meaningful coarse-grained models. In our approach, the cellular compartment is structured into a cytosolic region and a nucleoid region, and the processes of transcription and translation are assigned accordingly. The theoretical study reveals a quadratic relationship between the number of active ribosomes and the growth rate μ. While the overall available number of ribosomes follows the linear "bacterial growth law", the approach allows us to determine the growth limit for the chosen experimental environment (minimal medium, only one C source). The new approach is in good agreement with published experimental data, and, with a simple rule of thumb can be applied to other cellular systems.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:67 |
---|---|
Enthalten in: |
Metabolic engineering - 67(2021) vom: 01. Sept., Seite 145-152 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Kremling, Andreas [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 24.11.2021 Date Revised 24.11.2021 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1016/j.ymben.2021.06.006 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM32721502X |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM32721502X | ||
003 | DE-627 | ||
005 | 20231225200303.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.ymben.2021.06.006 |2 doi | |
028 | 5 | 2 | |a pubmed24n1090.xml |
035 | |a (DE-627)NLM32721502X | ||
035 | |a (NLM)34174424 | ||
035 | |a (PII)S1096-7176(21)00105-1 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Kremling, Andreas |e verfasserin |4 aut | |
245 | 1 | 2 | |a A counting-strategy together with a spatial structured model describes RNA polymerase and ribosome availability in Escherichia coli |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 24.11.2021 | ||
500 | |a Date Revised 24.11.2021 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2021 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved. | ||
520 | |a The allocation of resources during bacterial growth is strongly related to the availability of ribosomes and RNA polymerase molecules. Here, coarse-grained models offer a promising start due to their simple structure and the limited number of kinetic parameters. Based on published data sets for proteome and mRNA data in Escherichia coli, and together with mass balance equations describing gene expression, we are able to calculate the number of active molecules (that is, the number of ribosomes that are currently translating nascent and mature mRNA, as well as the number of RNA polymerase molecules on the DNA). This information is a prerequisite for meaningful coarse-grained models. In our approach, the cellular compartment is structured into a cytosolic region and a nucleoid region, and the processes of transcription and translation are assigned accordingly. The theoretical study reveals a quadratic relationship between the number of active ribosomes and the growth rate μ. While the overall available number of ribosomes follows the linear "bacterial growth law", the approach allows us to determine the growth limit for the chosen experimental environment (minimal medium, only one C source). The new approach is in good agreement with published experimental data, and, with a simple rule of thumb can be applied to other cellular systems | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Counting strategy | |
650 | 4 | |a Resource allocation | |
650 | 4 | |a Ribosome and RNA polymerase availability | |
650 | 4 | |a Spatial structured model | |
650 | 7 | |a Escherichia coli Proteins |2 NLM | |
650 | 7 | |a RNA, Messenger |2 NLM | |
650 | 7 | |a DNA-Directed RNA Polymerases |2 NLM | |
650 | 7 | |a EC 2.7.7.6 |2 NLM | |
773 | 0 | 8 | |i Enthalten in |t Metabolic engineering |d 1997 |g 67(2021) vom: 01. Sept., Seite 145-152 |w (DE-627)NLM097535249 |x 1096-7184 |7 nnns |
773 | 1 | 8 | |g volume:67 |g year:2021 |g day:01 |g month:09 |g pages:145-152 |
856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.ymben.2021.06.006 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 67 |j 2021 |b 01 |c 09 |h 145-152 |