Expression analysis and tissue localization of IgZ in the grouper Epinephelus coioides after Vibrio alginolyticus infection and vaccination
© 2021 John Wiley & Sons Ltd..
The orange-spotted grouper (Epinephelus coioides) is an important marine farmed fish in China. It is affected by the bacterial pathogen Vibrio alginolyticus, which causes high mortality and substantial economic losses. We studied the transcriptional changes of the IgZ gene in E. coioides following V. alginolyticus stimulation and investigated the distribution of IgZ in different tissues. The highest expression level of IgZ occurred in the head kidney. When fish were stimulated with live and inactivated V. alginolyticus, the expression levels of IgZ in the head kidney, spleen, intestine, gills and blood cells were significantly upregulated. In an in situ hybridization study, IgZ mRNA-positive cells were detected in the head kidney, spleen and gill, but positive signals were not detected in the liver and intestine. IgZ-labelled cells increased in the head kidney, spleen and gills post-infection with V. alginolyticus for 21 days. The present study provides additional evidence that IgZ is involved in mucosal immune responses and helps explain the role of IgZ in E. coioides defence against V. alginolyticus infection.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
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| Enthalten in: |
Journal of fish diseases - 44(2021), 10 vom: 07. Okt., Seite 1647-1655 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fu, Yao-Wu [VerfasserIn] |
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| Links: |
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| Themen: |
Bacterial Vaccines |
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| Anmerkungen: |
Date Completed 12.11.2021 Date Revised 12.11.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/jfd.13471 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM326813756 |
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| 245 | 1 | 0 | |a Expression analysis and tissue localization of IgZ in the grouper Epinephelus coioides after Vibrio alginolyticus infection and vaccination |
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| 500 | |a Date Revised 12.11.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 John Wiley & Sons Ltd. | ||
| 520 | |a The orange-spotted grouper (Epinephelus coioides) is an important marine farmed fish in China. It is affected by the bacterial pathogen Vibrio alginolyticus, which causes high mortality and substantial economic losses. We studied the transcriptional changes of the IgZ gene in E. coioides following V. alginolyticus stimulation and investigated the distribution of IgZ in different tissues. The highest expression level of IgZ occurred in the head kidney. When fish were stimulated with live and inactivated V. alginolyticus, the expression levels of IgZ in the head kidney, spleen, intestine, gills and blood cells were significantly upregulated. In an in situ hybridization study, IgZ mRNA-positive cells were detected in the head kidney, spleen and gill, but positive signals were not detected in the liver and intestine. IgZ-labelled cells increased in the head kidney, spleen and gills post-infection with V. alginolyticus for 21 days. The present study provides additional evidence that IgZ is involved in mucosal immune responses and helps explain the role of IgZ in E. coioides defence against V. alginolyticus infection | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Epinephelus coioides | |
| 650 | 4 | |a Vibrio alginolyticus | |
| 650 | 4 | |a IgZ | |
| 650 | 4 | |a Immunoglobulin | |
| 650 | 7 | |a Bacterial Vaccines |2 NLM | |
| 650 | 7 | |a Fish Proteins |2 NLM | |
| 650 | 7 | |a Immunoglobulin Heavy Chains |2 NLM | |
| 700 | 1 | |a Yao, Zhan-Juan |e verfasserin |4 aut | |
| 700 | 1 | |a He, Meng-Han |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zuo-Pei |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Wei-Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Cui, Miao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Qi-Zhong |e verfasserin |4 aut | |
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