Inhibitory Effect of Catechin-Rich Açaí Seed Extract on LPS-Stimulated RAW 264.7 Cells and Carrageenan-Induced Paw Edema

Açaí berry is a fruit from the tree commonly known as açaízeiro (Euterpe oleracea Mart.) originated from the Amazonian region and widely consumed in Brazil. There are several reports of the anti-inflammatory activity of its pulp and few data about the seed's potential in inflammation control. This work aimed to evaluate the effect of catechin-rich açaí extract on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and carrageenan-induced paw edema. The treatment with E. oleracea ethyl acetate extract (EO-ACET) was used in an in vitro model performed with macrophages stimulated by LPS, in which pro-inflammatory markers were evaluated, and in an in vivo model of acute inflammation, in which edema inhibition was evaluated. EO-ACET showed an absence of endotoxins, and did not display cytotoxic effects in RAW 264.7 cells. LPS-stimulated cells treated with EO-ACET displayed low levels of nitrite and interleukins (IL's), IL-1β, IL-6 and IL-12, when compared to untreated cells. EO-ACET treatment was able to inhibit carrageenan-induced paw edema at 500 and 1000 mg/kg, in which no acute inflammatory reaction or low mast cell counts were observed by histology at the site of inoculation of λ-carrageenan. These findings provide more evidence to support further studies with E. oleracea seeds for the treatment of inflammation.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:10

Enthalten in:

Foods (Basel, Switzerland) - 10(2021), 5 vom: 06. Mai

Sprache:

Englisch

Beteiligte Personen:

Xavier, Gabriel Silva [VerfasserIn]
Teles, Amanda Mara [VerfasserIn]
Moragas-Tellis, Carla Junqueira [VerfasserIn]
Chagas, Maria do Socorro Dos Santos [VerfasserIn]
Behrens, Maria Dutra [VerfasserIn]
Moreira, Wendel Fragoso de Freitas [VerfasserIn]
Abreu-Silva, Ana Lucia [VerfasserIn]
Calabrese, Kátia da Silva [VerfasserIn]
Nascimento, Maria do Desterro Soares Brandão [VerfasserIn]
Almeida-Souza, Fernando [VerfasserIn]

Links:

Volltext

Themen:

Cytokines
Euterpe oleracea
Fruit
Histology
Inflammation
Journal Article
Mast cells
Nitric oxide

Anmerkungen:

Date Revised 15.06.2021

published: Electronic

Citation Status PubMed-not-MEDLINE

doi:

10.3390/foods10051014

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM326149732